STUDIES ON DELAYED HYPERSENSITIVITY. I. INFERENCES ON THE COMPARATIVE BINDING AFFINITIES OF ANTIBODIES MEDIATING DELAYED AND IMMEDIATE HYPERSENSITIVITY REACTIONS IN THE GUINEA PIG.
STUDIES ON DELAYED HYPERSENSITIVITY. I. INFERENCES ON THE COMPARATIVE BINDING AFFINITIES OF ANTIBODIES MEDIATING DELAYED AND IMMEDIATE HYPERSENSITIVITY REACTIONS IN THE GUINEA PIG.
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DOI：
10.1084/jem.121.6.873
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发表时间：
1965-06-01
期刊：
影响因子：
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通讯作者：
LEVINE BB
中科院分区：
文献类型：
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作者：
LEVINE BB
在豚鼠中用几种明确的抗原系统（聚-L-赖氨酸和豚鼠血清白蛋白的半抗原结合物）进行的实验表明： 1. 阿蒂斯反应也表现出载体特异性，尽管程度比迟发型超敏反应小。 2.通过注射微量抗原进行脱敏导致迟发性超敏反应的中度特异性脱敏，而不会降低Arthus对相同抗原决定簇的反应性的脱敏。 3.由高亲和力豚鼠抗体制备的不溶性抗原抗体复合物可以在致敏豚鼠中引起特异性迟发性皮肤反应。 4.结构相似的半抗原的同源缀合物在延迟反应中表现出的交叉反应性比在对相同抗原决定簇的立即超敏反应中低得多。这些实验结果被解释为表明豚鼠的迟发型超敏反应是由相对高结合亲和力的“抗体”介导的。这些抗体的高结合亲和力更有可能是通过抗原和抗体之间更紧密的结构适应而不是通过更大的特异性接触面积来实现。
Experiments carried out with several well defined antigenic systems (hapten conjugates of poly-L-lysine and guinea pig serum albumin) in guinea pigs demonstrated that: 1. Arthus reactions also manifest carrier specificity, although to a smaller extent than do delayed hypersensitivity reactions. 2. Desensitization by injection of minute doses of antigen results in moderate specific desensitization of delayed hypersensitivity without desensitization of Arthus reactivity to the same antigenic determinant. 3. Insoluble antigen-antibody complexes prepared from high affinity guinea pig antibodies can elicit specific delayed skin reactions in sensitized guinea pigs. 4. Homologous conjugates of structurally similar haptens show considerably less cross-reactivity in delayed reactions than in immediate hypersensitivity reactions to the same antigenic determinant. These experimental results are interpreted as indicating that delayed hypersensitivity reactions in the guinea pig are mediated by "antibodies" of comparatively high binding affinities. High binding affinities are achieved for these antibodies more likely by closer structural adaptation between antigen and antibody than by a larger area of specific contact.