Recruitment of heterogeneous nuclear ribonucleoprotein A1 in vivo to the LMP/TAP region of the major histocompatibility complex
Recruitment of heterogeneous nuclear ribonucleoprotein A1 in vivo to the LMP/TAP region of the major histocompatibility complex
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DOI：
10.1074/jbc.m206621200
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发表时间：
2003-02-14
影响因子：
4.8
通讯作者：
Sheer, D
中科院分区：
文献类型：
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作者：
Donev, R;Horton, R;Sheer, D
在人类和小鼠主要组织相容性复合体II类区域中的LMP/TAP基因簇附近被鉴定。这些序列显示出作为核基质附着区（MAR）的功能。五个人MAR中的三个和单个小鼠MAR在体内主要组织相容性复合体11类基因的转录上调过程中招募异质核核糖核蛋白A1（hnRNP-A1）。这种募集的时间与成熟TAP 1 mRNA的升高相关。两个人NLARs在体外直接结合hnRNP-A1，其35-bp序列与某些Alu重复序列具有超过90%的相似性。这项研究表明，MAR招募和结合hnRNP-A1转录上调。
were identified adjacent to the LMP/TAP gene cluster in the human and mouse major histocompatibility complex class II region. These sequences were shown to function as nuclear matrix attachment regions (MARs). Three of the five human MARs and the single mouse MAR recruit heterogeneous nuclear ribonucleoprotein A1 (hnRNP-A1) in vivo during transcriptional up-regulation of the major histocompatibility complex class 11 genes. The timing of this recruitment correlates with a rise in mature TAP1 mRNA. Two of the human NLARs bind hnRNP-A1 in vitro directly within a 35-bp sequence that shows over 90% similarity to certain Alu repeat sequences. This study shows that MARs recruit and bind hnRNP-A1 upon transcriptional up-regulation.