Locomotor activity, ultrasonic vocalization and oxytocin levels in infant CD38 knockout mice
Locomotor activity, ultrasonic vocalization and oxytocin levels in infant CD38 knockout mice
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DOI：
10.1016/j.neulet.2008.09.084
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发表时间：
2008-12-19
影响因子：
2.5
通讯作者：
Higashida, Haruhiro
中科院分区：
文献类型：
--
作者：
Liu, Hong-Xiang;Lopatina, Olga;Higashida, Haruhiro
催产素 (OT) 是一种参与生殖的神经激素，在从啮齿动物到人类的多种哺乳动物的社会行为中发挥着至关重要的作用。 CD38 在调节 OT 分泌以促进社会行为方面的作用已在成年小鼠中得到证实，但尚未在幼鼠或发育过程中进行检查。与母亲分离会给 7 天大的幼鼠带来压力。在这种分离过程中，CD38 敲除 (CD38(+/-)) 幼崽的运动活性高于野生型 (CD38(+/-)) 或杂合子 (CD38(+/-)) 对照。 CD38(-/-)幼犬的超声波发声次数低于CD38(+/+)幼犬。然而，两种基因型之间的差异没有 OT 敲除或 OT 受体敲除小鼠严重。为了解释这一点，我们测量了血浆OT水平，CD38(-/-)幼崽在出生后1-3周期间该水平并不低，但在断奶后(>3周)显着降低。 CD38(-/-)小鼠下丘脑和垂体中的ADP-核糖基环化酶活性从出生后1周开始显着降低，并且此后一直降低到成年阶段（2个月大）。这些结果表明，CD38(-/-)幼崽行为异常严重程度的降低是由于高水平血浆OT的部分补偿所致。 (c) 2008 Elsevier Ireland Ltd. 保留所有权利。
Oxytocin (OT), a neurohormone involved in reproduction, plays a critical role in social behavior in a wide range of mammalian species from rodents to humans. The role of CD38 in regulating OT secretion for social behavior has been demonstrated in adult mice, but has not been examined in pups or during development. Separation from the dam induces stress in 7-day-old mouse pups. During such isolation, locomotor activity was higher in CD38 knockout (CD38(+/-)) pups than in wild-type (CD38(+/-)) or heterozygous (CD38(+/-)) controls. The number of ultrasonic vocalizations was lower in CD38(-/-) pups than in CD38(+/+) pups. However, the difference between the two genotypes was less severe than that in OT knockout or OT receptor knockout mice. To explain this, we measured plasma OT levels, The level was not lower in CD38(-/-) pups during the period 1-3 weeks after birth, but was significantly reduced after weaning (> 3 weeks). ADP-ribosyl cyclase activities in the hypothalamus and pituitary were markedly lower from 1 week after birth in CD38(-/-) mice and were consistently lower thereafter to the adult stage (2 months old). These results showed that the reduced severity of behavioral abnormalities in CD38(-/-) pups was due to partial compensation by the high level of plasma OT. (c) 2008 Elsevier Ireland Ltd. All rights reserved.