The proteome of rat olfactory sensory cilia
The proteome of rat olfactory sensory cilia
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DOI：
10.1002/pmic.200800149
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发表时间：
2009-01-01
期刊：
影响因子：
3.4
通讯作者：
Moehrlen, Frank
中科院分区：
文献类型：
--
作者：
Mayer, Ulrich;Kueller, Alexander;Moehrlen, Frank
嗅感觉神经元暴露于吸入的空气化学感觉纤毛，其结合气味物质并作为转导细胞器操作。睫状膜中的气味受体激活转导级联，其使用cAMP和Ca 2+用于睫状腔中的感觉信号传导。虽然经典的转导途径已经建立，但感觉转导的更复杂方面的分子组分，如受体反应的适应、调节和终止，尚未系统地鉴定。此外，嗅觉生理学中的未决问题包括纤毛如何与周围的粘液交换溶质，组装高度极化的蛋白质组，以及科普环境空气中的有害物质。一个特定的纤毛蛋白质组将促进在所有这些领域的研究工作。我们改进了从大鼠嗅感觉神经元分离纤毛的方法，并分离出了一种特异性富集纤毛膜蛋白的制剂。利用LC-ESI-MS/MS分析，我们确定了377个蛋白质组成的嗅纤毛蛋白质组。这些蛋白质代表了嗅觉研究的全面数据集，因为超过80%可以归因于嗅觉感觉神经元及其纤毛的特征功能：信号处理，蛋白质靶向，神经发生，溶质转运和细胞保护。因此，细胞器蛋白质组学产生了决定性的信息，不同的生理功能的感觉细胞器。
Olfactory sensory neurons expose to the inhaled air chemosensory cilia which bind odorants and operate as transduction organelles. Odorant receptors in the ciliary membrane activate a transduction cascade which uses cAMP and Ca2+ for sensory signaling in the ciliary lumen. Although the canonical transduction pathway is well established, molecular components for more complex aspects of sensory transduction, like adaptation, regulation, and termination of the receptor response have not been systematically identified. Moreover, open questions in olfactory physiology include how the cilia exchange solutes with the surrounding mucus, assemble their highly polarized set of proteins, and cope with noxious substances in the ambient air. A specific ciliary proteome would promote research efforts in all of these fields. We have improved a method to detach cilia from rat olfactory sensory neurons and have isolated a preparation specifically enriched in ciliary membrane proteins. Using LC-ESI-MS/MS analysis, we identified 377 proteins which constitute the olfactory cilia proteome. These proteins represent a comprehensive data set for olfactory research since more than 80% can be attributed to the characteristic functions of olfactory sensory neurons and their cilia: signal processing, protein targeting, neurogenesis, solute transport, and cytoprotection. Organellar proteomics thus yielded decisive information about the diverse physiological functions of a sensory organelle.