A novel collagen/hydroxyapatite/poly(lactide-co-ε-caprolactone) biodegradable and bioactive 3D porous scaffold for bone regeneration
A novel collagen/hydroxyapatite/poly(lactide-co-ε-caprolactone) biodegradable and bioactive 3D porous scaffold for bone regeneration
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DOI：
10.1002/jbm.a.33033
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发表时间：
2011-03-15
影响因子：
4.9
通讯作者：
Rouabhia, Mahmoud
中科院分区：
文献类型：
--
作者：
Akkouch, Adil;Zhang, Ze;Rouabhia, Mahmoud
本研究的目的是使用胶原（科尔）、羟基磷灰石（HA）和聚（丙交酯-ε-己内酯）（PLCL）设计一种具有适合骨工程的组成和微观结构的无毒支架。矿化I型科尔是通过HA颗粒在自组装科尔纤维内直接成核而产生的，以获得科尔/HA复合物，然后将其加入到溶解在1，4-二氧六环中的PLCL（70：30）中。然后通过冷冻干燥/冻干和盐浸法制备三维多孔科尔/HA/PLCL支架。得到的科尔/HA/PLCL支架显示出高度均匀的孔隙率和高度互连的孔。X射线光电子能谱仪和傅立叶变换红外分析显示，胶原和HA颗粒的存在下，表面上的科尔/HA/PLCL支架。增殖试验、显微镜观察和定量RT-PCR基因分析表明，成骨细胞能够在科尔/HA/PLCL支架上附着、增殖并保持成骨细胞样表型。总之，我们制备了含有天然聚合物（科尔和HA）和合成聚合物（PLCL）的无毒支架。通过其化学组成和多孔形态，这种支架可能对成骨细胞生长、分化和骨组织形成有用。(C)2011 Wiley Periodicals，Inc. J Biomed Mater Res Part A：96A：693-704，2011.
The goal of this study was to design a nontoxic scaffold with both composition and microstructure suitable for bone engineering using collagen (Coll), hydroxyapatite (HA), and poly(lactide-co-epsilon-caprolactone) (PLCL). Mineralized type I Coll was produced by direct nucleation of HA particles inside self-assembled Coll fibers to obtain a Coll/HA complex, which was then added to dissolved PLCL (70: 30) in 1,4-dioxane. A 3D porous Coll/HA/PLCL scaffold was subsequently produced through freeze-drying/lyophilization and salt-leaching procedures. The resulting Coll/HA/PLCL scaffold displayed a high uniform porosity and highly interconnected pores. X-ray photoelectron spectrometer and Fourier transform infrared analyses revealed the presence of both collagen and HA particles on the surface of the Coll/HA/PLCL scaffold. Proliferation assay, microscopic observations, and gene analysis with quantitative RT-PCR showed that osteoblast cells were able to attach, proliferate, and maintain an osteoblastlike phenotype when cultured on the Coll/HA/PLCL scaffold. In summary, we produced a nontoxic scaffold that contains natural polymers (Coll and HA) and synthetic polymer (PLCL). Through its chemical composition and porous morphology, this scaffold may be useful for osteoblast growth, differentiation, and bone tissue formation. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 96A: 693-704, 2011.