ASSESSMENT OF THE KIDNEY-FUNCTION IN MAINTENANCE OF PLASMA GLUTATHIONE CONCENTRATION AND REDOX STATE IN ANESTHETIZED RATS
ASSESSMENT OF THE KIDNEY-FUNCTION IN MAINTENANCE OF PLASMA GLUTATHIONE CONCENTRATION AND REDOX STATE IN ANESTHETIZED RATS
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DOI：
10.1016/0014-5793(79)80558-x
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发表时间：
1979-01-01
期刊：
影响因子：
3.5
通讯作者：
LACHENMAIER, C
中科院分区：
文献类型：
--
作者：
HABERLE, D;WAHLLANDER, A;LACHENMAIER, C
已经提供了大量证据证明肾脏在细胞外谷胱甘肽代谢周转中的作用[1 -12]。下午3-5点，在大鼠动脉血浆[7，13]和人静脉血浆[141]的细胞外间隙中发现了谷氨酸盐。在对细胞外谷胱甘肽命运的研究中，已经提出降解主要发生在肾刷状缘膜的腔表面，并且γ-谷氨酰转肽酶是作用于细胞外谷胱甘肽的谷氨酰胺酶。然而，未对完整动物的肾酶功能进行定量评估。为了提供关于这一假设的更多信息，我们测量了麻醉大鼠171肾脏的谷胱甘肽摄取。有趣的是，肾谷氨酰胺提取率为~ 90%。鉴于肾滤过分数约为30%，这表明，除了小管内降解外，肾脏还必须有进一步的谷胱甘肽摄取机制，根据-缩略语：CSH，还原型谷胱甘肽; GSSG，谷胱甘肽二硫化物
Substantial evidence has been provided for a role of the kidney in the metabolic turnover of extracellular glutathione [l-12]. Glutathione has been found in the extracellular space at 3-5 PM in the arterial plasma of the rat [7, 13] and in human venous plasma [141. In studies of the fate of extracellular glutathione the degradation has been proposed to occur mainly on the luminal surface of the renal brush-border membrane and that the y-glutamyl transpeptidase is a gluta~ ionase acting on extracellular glutathione. However, a quantitative assessment of this function of the kidney enzyme in the intact animal was not performed. In order to provide more information on this hypothesis, we measured glutathione uptake by the kidney in the anaesthetized rat 171. Interestingly, renal glutatl~ ione extraction was-90%. In view of the renal Rltration fraction of-30% this indicated that, in addition to the intratubular degradation, there must be a further uptake mechanism for glutathione by the kidney, in accor-Abbreviations: CSH, reduced glutathione; GSSG, glutathione disulfide