Noncontact depth-resolved micro-scale optical coherence elastography of the cornea.
Noncontact depth-resolved micro-scale optical coherence elastography of the cornea.
复制标题
DOI：
10.1364/boe.5.003807
复制
发表时间：
2014-11
影响因子：
3.4
通讯作者：
Shang Wang;K. Larin
中科院分区：
文献类型：
--
作者：
Shang Wang;K. Larin
高分辨率的角膜弹性成像评估可以极大地帮助临床诊断和治疗各种眼科疾病。在这里，我们报告的第一个非接触式深度分辨微尺度光学相干弹性成像的角膜实现剪切波成像光学相干断层扫描（SWI-OCT）结合角膜兰姆波传播的光谱分析。这种成像方法依赖于聚焦的喷气装置，以高度局部化的低压短时间气流加载角膜，并应用相位分辨OCT检测来捕获具有纳米级灵敏度的低幅度变形。SWI-OCT系统在此用于以与OCT A线采集速度相同的帧率对角膜兰姆波传播进行成像。通过对角膜变形轮廓的频谱分析，得到了不同深度处Lamb波主要频率分量的相速度，揭示了角膜硬度与其结构特征的相关性。我们对离体兔眼的初步实验证明了该方法在角膜深度分辨微观弹性成像中的可行性。兰姆波色散的评估也提出了，这表明角膜粘弹性的定量测量的潜力。
High-resolution elastographic assessment of the cornea can greatly assist clinical diagnosis and treatment of various ocular diseases. Here, we report on the first noncontact depth-resolved micro-scale optical coherence elastography of the cornea achieved using shear wave imaging optical coherence tomography (SWI-OCT) combined with the spectral analysis of the corneal Lamb wave propagation. This imaging method relies on a focused air-puff device to load the cornea with highly-localized low-pressure short-duration air stream and applies phase-resolved OCT detection to capture the low-amplitude deformation with nano-scale sensitivity. The SWI-OCT system is used here to image the corneal Lamb wave propagation with the frame rate the same as the OCT A-line acquisition speed. Based on the spectral analysis of the corneal temporal deformation profiles, the phase velocity of the Lamb wave is obtained at different depths for the major frequency components, which shows the depthwise distribution of the corneal stiffness related to its structural features. Our pilot experiments on ex vivo rabbit eyes demonstrate the feasibility of this method in depth-resolved micro-scale elastography of the cornea. The assessment of the Lamb wave dispersion is also presented, suggesting the potential for the quantitative measurement of corneal viscoelasticity.