Retrogradely transported CTB-saporin kills sympathetic preganglionic neurons
Retrogradely transported CTB-saporin kills sympathetic preganglionic neurons
复制标题
DOI：
10.1097/00001756-199902050-00019
复制
发表时间：
1999-02-05
期刊：
影响因子：
1.7
通讯作者：
Minson, JB
中科院分区：
文献类型：
--
作者：
Llewellyn-Smith, IJ;Martin, CL;Minson, JB
为了切除支配某一特定靶点的交感神经节前神经元（SPN），我们将霍乱毒素B亚基（CTB）偶联皂苷单侧注入大鼠颈上神经节。注射后3 d，脊髓t1 ~ t3节段注射神经节同侧和对侧中外侧细胞柱胆碱能神经元数量有显著差异。第14天，77%的同侧胆碱能神经元消失。T1-T2组神经元死亡比例高于T3组。对比注射CTB皂苷大鼠的SPN计数和未结合CTB注入颈上神经节的大鼠的SPN计数表明，供应该神经节的84%的SPN在14天内死亡。逆行转运的CTB皂苷杀死交感神经节前神经元，也可能消除转运CTB的其他类型神经元。(C) 1999 Lippincott Williams & Wilkins。
AIMING to ablate sympathetic preganglionic neurons (SPN) innervating a defined target, we injected saporin conjugated to cholera toxin B subunit (CTB) unilaterally into the superior cervical ganglion of rats. In spinal cord segments T1-T3, the numbers of cholinergic neurons in the intermediolateral cell column ipsilateral and contralateral to the injected ganglion were significantly different by 3 days post-injection. By day 14, 77% of ipsilateral cholinergic neurons had disappeared. A higher percentage of neurons were killed in T1-T2 than in T3. Comparing SPN counts from CTB-saporin injected rats and counts from rats receiving unconjugated CTB into the superior cervical ganglion indicated that 84% of SPN supplying the ganglion had died by 14 days. Retrogradely transported CTB-saporin kills sympathetic preganglionic neurons and may also eliminate other types of neurons that transport CTB. (C) 1999 Lippincott Williams & Wilkins.