High-frequency generalised transduction by bacteriophage T4
High-frequency generalised transduction by bacteriophage T4
复制标题
噬菌体 T4 的高频广义转导
作者：
G. Wilson;K. Young;G. Edlin;W. Konigsberg
广义转导--通过噬菌体将细菌基因从一个细胞转移到另一个细胞--是细菌获得外源遗传信息的少数过程之一。Zinder和Lederberg1，2首先用噬菌体P22在鼠伤寒沙门氏菌中证实了转导，随后在大肠杆菌中用噬菌体P13和T14证实了转导。噬菌体T4是最大的噬菌体，也是研究最彻底的毒力噬菌体之一，但从未被观察到介导普遍的转导。在这份报告中，我们描述了T4的一个多重突变体，它表现出普遍转导的特性，并以通常比其他转导噬菌体更高的频率转移大肠杆菌基因。
GENERALISED transduction—the transfer of bacterial genes from one cell to another via bacteriophages—is one of a small number of processes by which bacteria can acquire exogenous genetic information. Transduction was first demonstrated in Salmonella typhimurium with the phage P22 by Zinder and Lederberg1,2 and subsequently in Escherichia coli with the phages P13 and T14. Bacteriophage T4 is the largest, and one of the most thoroughly studied of the virulent coliphages but has never been observed to mediate generalised transduction. In this report we describe a multiple mutant of T4 that displays the property of generalised transduction, and transfers E. coli genes with frequencies that, in general, are higher than those observed for other transducing phages.