A 'rare biosphere' microorganism contributes to sulfate reduction in a peatland.
A 'rare biosphere' microorganism contributes to sulfate reduction in a peatland.
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DOI：
10.1038/ismej.2010.75
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发表时间：
2010-12
期刊：
影响因子：
--
通讯作者：
Loy A
中科院分区：
文献类型：
--
作者：
Pester M;Bittner N;Deevong P;Wagner M;Loy A
泥炭地的甲烷排放对全球变暖有很大的贡献，但硫酸盐还原显着减少，这是由全球不断增加的空气硫污染所推动的。然而，陆地生态系统中硫酸盐还原背后的生物学还没有得到很好的理解，这一过程的关键参与者及其丰度仍然没有确定。比较16 S rRNA基因的稳定同位素探测在存在和不存在的硫酸盐表明，Desulfosporosinus物种，其中仅占0.006%的总微生物群落16 S rRNA基因，是一个重要的硫酸盐还原剂在长期的实验泥炭地现场。使用dsrAB [编码异化（亚）硫酸盐还原酶的亚基A和B]的平行稳定同位素探测在测试条件下未鉴定出另外的硫酸盐还原剂。对于已鉴定的Desulfosporosinus物种，估计高细胞特异性硫酸盐还原速率高达341 fmol SO 42 − cell−1 day−1。因此，小Desulfosporosinus种群具有以4.0-36.8 nmol（g土壤w.重量）-1天-1，足以说明泥炭土中硫酸盐还原的相当大一部分。硫酸盐扩散到这种高活性细胞的建模表明，即使在低至10 μM的本体浓度下，硫酸盐供应也没有限制。总的来说，这些数据表明，已确定的Desulfosporosinus物种，尽管是“稀有生物圈”的成员，有助于一个重要的地球化学过程，将泥炭地的碳流从甲烷转移到二氧化碳，从而改变了它们对全球变暖的贡献。
Methane emission from peatlands contributes substantially to global warming but is significantly reduced by sulfate reduction, which is fuelled by globally increasing aerial sulfur pollution. However, the biology behind sulfate reduction in terrestrial ecosystems is not well understood and the key players for this process as well as their abundance remained unidentified. Comparative 16S rRNA gene stable isotope probing in the presence and absence of sulfate indicated that a Desulfosporosinus species, which constitutes only 0.006% of the total microbial community 16S rRNA genes, is an important sulfate reducer in a long-term experimental peatland field site. Parallel stable isotope probing using dsrAB [encoding subunit A and B of the dissimilatory (bi)sulfite reductase] identified no additional sulfate reducers under the conditions tested. For the identified Desulfosporosinus species a high cell-specific sulfate reduction rate of up to 341 fmol SO42− cell−1 day−1 was estimated. Thus, the small Desulfosporosinus population has the potential to reduce sulfate in situ at a rate of 4.0–36.8 nmol (g soil w. wt.)−1 day−1, sufficient to account for a considerable part of sulfate reduction in the peat soil. Modeling of sulfate diffusion to such highly active cells identified no limitation in sulfate supply even at bulk concentrations as low as 10 μM. Collectively, these data show that the identified Desulfosporosinus species, despite being a member of the ‘rare biosphere’, contributes to an important biogeochemical process that diverts the carbon flow in peatlands from methane to CO2 and, thus, alters their contribution to global warming.
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影响因子：
9.8
作者：
Alewell, C.;Paul, S.;Storck, F. R.
通讯作者：
Storck, F. R.
DOI：
10.1073/pnas.0404412101
发表时间：
2004-08-24
影响因子：
11.1
作者：
Gauci, V;Matthews, E;Vile, M
通讯作者：
Vile, M
影响因子：
4.4
作者：
Detmers, J;Br端chert, V;Kuever, J
通讯作者：
Kuever, J
影响因子：
4.4
作者：
Houweling, S;Kaminski, T;Heimann, M
通讯作者：
Heimann, M
影响因子：
8.9
作者：
Alewell, C;Novak, M
通讯作者：
Novak, M