Brain Polymorphonuclear Leukocyte Quantitation by Peroxidase Assay
Brain Polymorphonuclear Leukocyte Quantitation by Peroxidase Assay
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通过过氧化物酶测定进行脑多形核白细胞定量
DOI：
10.1128/iai.10.2.356-360.1974
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发表时间：
1974
影响因子：
3.1
通讯作者：
D. Smith
中科院分区：
文献类型：
--
作者：
A. Smith;I. Rosenberg;D. Averill;E. Moxon;T. Stossel;D. Smith
通过分光光度法测定髓过氧化物酶活性来定量大鼠脑多形核浸润。通过在过氧化氢生成系统（葡萄糖-葡萄糖氧化酶）存在下邻联茴香胺的氧化来测定过氧化物酶。酶活性与密度为 105 至 106 个细胞的多形核白细胞数量成正比。脑组织没有可检测到的过氧化物酶活性，并且对添加到脑匀浆中的多形核白细胞进行了定量估计。在患有流感嗜血杆菌脑膜炎的幼年大鼠中，通过过氧化物酶活性测量发现的脑中多形核白细胞数量与独立组织学分级之间的相关性并不精确，但在预测多形核白细胞存在与否方面存在确切的相关性。髓过氧化物酶活性似乎为定量测定组织多形核白细胞含量提供了一种快速方便的工具。
Rat brain polymorphonuclear infiltration was quantitated by spectrophotometric assay of myeloperoxidase activity. Peroxidase was assayed by following the oxidation of o-dianisidine in the presence of a hydrogen peroxide generating system (glucose-glucose oxidase). Enzyme activity was proportional to the number of polymorphonuclear leukocytes present at densities between 105 and 106 cells. Brain tissue did not have detectable peroxidase activity and polymorphonuclear leukocytes added to brain homogenates were quantitatively estimated. In infant rats with Haemophilus influenzae meningitis, the correlation between the number of polymorphonuclear leukocytes found in brain by peroxidase activity measurement and independent histological grading was not precise, but there was an exact correlation in predicting presence or absence of polymorphonuclear leukocytes. Myeloperoxidase activity appears to afford a rapid convenient tool for quantitative determination of tissue polymorphonuclear leukocyte content.