Behaviour of the disease resistance gene Asc in protoplasts of Lycopersicon esculentum mill
Behaviour of the disease resistance gene Asc in protoplasts of Lycopersicon esculentum mill
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抗病基因Asc在番茄原生质体中的行为
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
D. Gilchrist
中科院分区：
文献类型：
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作者：
V. Moussatos;H. Witsenboer;J. Hille;D. Gilchrist
摘要在单个细胞水平上检测到ASC的作用，这是一个控制整个植物水平上疾病反应的单一显性孟德尔基因。从抗性(ASC/ASC)和感病(ASC/ASC)番茄品系中新分离的原生质体对AAL毒素具有不同的敏感性。两个品系的原生质体死亡依赖于毒素浓度，所需时间与小叶组织的坏死时间相同，但Asc/Asc原生质体的敏感度比相应的小叶组织低10倍(0.15μM)，而Asc/Asc原生质体的敏感度(>；150μM)低100多倍。小叶的坏死需要光照，但原生质体不需要光照；ASC/ASC小叶在100倍的过量毒素浓度下在黑暗中保持48h绿色，而原生质体在光或暗条件下表现出同等的敏感性。衰老降低了小叶和原生质体对毒素的敏感性。完全扩张的ASC/ASC小叶对AAL毒素(∼30%坏死)的敏感性不到快速扩张小叶(∼87%坏死)的一半，而相同小叶的原生质体在分离后2天对AAL毒素的敏感性仅为80%，9天后对AAL毒素不敏感。这些数据表明，ASC基因的作用在原生质体中表达，细胞死亡的过程在单细胞和完整的叶片中类似，但在分化组织中表达的额外过程可以改变ASC对AAL毒素的反应。
Abstract Action of Asc , a single dominant Mendelian gene controlling disease response at the whole plant level, was detected at the level of individual cells. Protoplasts, freshly isolated from resistant ( Asc/Asc ) and susceptible ( asc/asc ) tomato isolines, were differentially sensitive to AAL toxin as observed in planta . Protoplast mortality was toxin concentration dependent in both isolines and required the same 24-36 h time frame as necrosis in leaflet tissue, but asc/asc protoplasts were 10-fold less sensitive (0.15 μM) and Asc/Asc protoplasts were over 100-fold less sensitive (> 150 μM) than corresponding leaflet tissue. Light was required for necrosis in leaflets but not in protoplasts; asc/asc leaflets kept in darkness remained green for 48 h at 100-fold excess toxin concentration whereas protoplasts showed equivalent sensitivity in light or dark. Aging diminished toxin sensitivity in both leaflets and in protoplasts. Fully expanded asc/asc leaflets were less than half as sensitive to AAL toxin (∼ 30% necrosis) as rapidly expanding leaflets (∼ 87% necrosis) while protoplasts from equivalent leaflets were only 80% as sensitive to AAL toxin 2 days after isolation and insensitive after 9 days. These data indicated that the action of the Asc gene is expressed in protoplasts, the process of cell death is analogous in single cells and intact leaves, but that additional processes expressed in differentiated tissue can modify Asc response to AAL-toxin.