EFFECT OF PHENOLIC ANTIBACTERIAL AGENT FENTICHLOR ON ENERGY COUPLING IN STAPHYLOCOCCUS-AUREUS
EFFECT OF PHENOLIC ANTIBACTERIAL AGENT FENTICHLOR ON ENERGY COUPLING IN STAPHYLOCOCCUS-AUREUS
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DOI：
10.1111/j.1365-2672.1974.tb00422.x
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发表时间：
1974-01-01
期刊：
影响因子：
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通讯作者：
BLOOMFIELD, SF
中科院分区：
文献类型：
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作者：
BLOOMFIELD, SF
实验证据表明，抑制主动转运是芬草胺对金黄色葡萄球菌的抑菌作用的原因。在有氧和无氧条件下，Fentichlor抑制谷氨酸的主动运输和同化以及葡萄糖的同化，但其同化抑制作用主要是通过抑制积累而不是直接作用于同化过程。在不存在葡萄糖的情况下，芬草胺消散膜质子梯度和ATP池，但是，在存在葡萄糖的情况下，ATP合成不受影响，并且尽管质子移位发生一些加速，但是仅导致梯度的部分消散。结果表明，芬草胺介导的质子易位阻止了膜质子运动与化学渗透假说所假设的主动转运的偶联。芬草胺部分抑制糖酵解和厌氧ATP合成，这可能有助于在厌氧条件下抑菌。
Summary.Experimental evidence indicates that inhibition of active transport is responsible for the bacteriostatic action of Fentichlor againstStaphylococcus aureus. Fentichlor inhibits active transport and assimilation of glutamic acid, and assimilation of glucose under aerobic and anaerobic conditions, but inhibition of assimilation results mainly from inhibition of accumulation rather than direct action on the assimilation process. In the absence of glucose, Fentichlor dissipates the membrane proton gradient and ATP pool but, with glucose present, ATP synthesis is unaffected and although some acceleration of proton translocation occurs, only partial dissipation of the gradient results. Results indicate that Fentichlor‐mediated proton translocation prevents coupling of membrane proton movements to active transport as postulated by the chemiosmotic hypothesis. Fentichlor partially inhibits glycolysis and anaerobic ATP synthesis which may contribute to bacteriostasis under anaerobic conditions.