New Insight into Anopheles coluzzii Coetzee & Wilkerson, 2013 larval ecology in the Wouri estuary, Littoral-Cameroon.
New Insight into Anopheles coluzzii Coetzee & Wilkerson, 2013 larval ecology in the Wouri estuary, Littoral-Cameroon.
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DOI：
10.1007/s13149-016-0519-9
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发表时间：
2017-05-01
影响因子：
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通讯作者：
Mimpfoundi, R.
中科院分区：
文献类型：
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作者：
Mbida, A. Mbida;Etang, J.;Mimpfoundi, R.
疟疾病媒的控制基本上是基于对成年蚊子使用杀虫剂。然而，由于对杀虫剂的抗药性的发展，现在人们对幼虫来源的管理重新产生了兴趣。本研究的目的是绘制和描述喀麦隆乌里河河口库鲁兹按蚊的繁殖地。2013年12月至2014年8月，在马诺卡岛的农村地区和杜阿拉的尤普韦市区进行了幼虫调查。对确定的库蚊科繁殖地进行了地理参照和地图绘制。幼虫生产力测定采用“浸泡”法，理化参数测定采用分光光度法和血氧法。收集到的蚊科幼虫在昆虫室内饲养至成虫阶段。成年蚊子进行了形态鉴定，属于冈比亚按蚊复合体的蚊子随后通过聚合酶链式反应-限制性片段长度多态性技术进行了分子鉴定。这两个地点共有240个繁殖点，包括10个类型。废弃的容器和水池分别是马诺卡和尤普韦最常见的繁殖地点。经形态和分子鉴定，共鉴定出蚊虫11种。库仑按蚊和致倦库蚊分别是马诺卡和尤布韦最常见的种类。在Manoka和Youpwe，管理的排水沟和独木舟上的蚊子密度分别较高。库蚊和伊蚊分别在马诺卡和尤普韦的中空棕榈井和水井中较常见。繁殖地点的生产力因理化参数不同而不同。物种丰富度因繁殖地的类型而异。喀麦隆首次在储水容器、轮胎、废弃容器和独木舟中发现了库鲁兹按蚊。这项研究突出了蚊子繁殖地点类型的多样性。Coluzzii在乌里河口，表明该物种适应其环境。这些结果可用于在马诺卡和尤普韦制定抗幼虫防治策略。
Malaria vectors control is essentially based on the use of insecticides against adult mosquitoes. However because of the development of resistance to insecticides, there is now a renewed interest in the management of larval sources. The aim of the present study was to map and characterize the breeding sites of Anopheles coluzzii in the Wouri river estuary in Cameroon. Larval surveys were carried out between December 2013 and August 2014 in rural areas on the island of Manoka and urban area in Youpwe at Douala. Culicidae breeding sites identified were geo-referenced and mapped. Their larval productivity was evaluated by the method of "dipping" and their physicochemical parameters measured by spectrophotometry and oximetry. Culicidae collected larvae were reared in the insectarium to the adult stage. Adult mosquitoes were subjected to morphological identification and those belonging to the Anopheles gambiae complex have subsequently been subjected to molecular identification by the PCR-RFLP technique. A total of 240 breeding sites were geo-referenced in the two sites, including 10 types. Abandoned containers and pools were the most frequent breeding sites respectively in Manoka and in Youpwe. After morphological and molecular identification, eleven mosquito species have been identified. Anopheles coluzzii and Culex quinquefasciatus were the most frequent species respectively in Manoka and in Youpwe. Mosquito density was higher in managed gutters and canoes respectively in Manoka and in Youpwe. Culex and Aedes genus were more frequent in the hollow palm and water wells respectively in Manoka and Youpwe. The productivity of breeding sites varied according to the physicochemical parameters. Species richness varied according to the type of breeding site. Anopheles coluzzii was observed for the first time in Cameroon in water storage containers, tires, discarded containers and canoes. This study highlighted diversity in the type of breeding site of An. coluzzii in the Wouri estuary, suggesting the adaptation of this species in its environment. These results could be used to develop an antilarval control strategy in Manoka and in Youpwe.