The presence of antibodies to oxidative modified proteins in serum from polycystic ovary syndrome patients
The presence of antibodies to oxidative modified proteins in serum from polycystic ovary syndrome patients
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DOI：
10.1111/j.1365-2249.2006.03061.x
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发表时间：
2006-05-01
影响因子：
4.6
通讯作者：
Martínez, P
中科院分区：
文献类型：
--
作者：
Palacio, JR;Iborra, A;Martínez, P
多囊卵巢综合征（PCOS）影响5-10%的育龄妇女。自由基作为氧化应激的产物，损害与人类生育有关的细胞和组织特性。这些自由基与氧化分子一起，可能对精子和卵母细胞、早期胚胎发育或子宫内膜产生细胞毒性或有害作用。醛修饰蛋白具有高度的免疫原性，针对新表位（如丙二醛（MDA））的循环自身抗体可能影响生殖系统。血清中自身抗体或活性氧（ROS）升高常与炎症反应有关。本研究的目的是研究PCOS患者血清中氧化蛋白（蛋白- mda）和抗子宫内膜抗体（AEA）水平是否高于对照组，并确定AEA特异性是否与氧化蛋白衍生物有关。选取31例因不孕症就诊的女性患者血清[10例PCOS患者（PCOS组）和21例男性因素导致不孕症的女性（对照组）]。采用酶联免疫吸附法（ELISA）检测子宫内膜细胞系（RL-95）的抗子宫内膜抗体。ELISA法检测抗MDA修饰的人血清白蛋白（HSA-MDA）抗体。用比色法测定血清中氧化蛋白（蛋白- mda）含量。PCOS患者血清中AEA、抗hsa - mda及氧化蛋白（蛋白- mda）水平明显高于对照组。第一次，我们描述了多囊卵巢综合征患者的自身免疫反应，在AEA方面。这些患者血清中存在蛋白- mda，加之体外对mda修饰蛋白（HSA-MDA）的抗体反应性增高，支持氧化应激可能是PCOS患者子宫内膜环境异常、胚胎接受性差的重要原因之一。
Polycystic ovary syndrome (PCOS) affects 5-10% of women of reproductive age. Free radicals, as a product of oxidative stress, impair cells and tissue properties related to human fertility. These free radicals, together with the oxidized molecules, may have a cytotoxic or deleterious effects on sperm and oocytes, on early embryo development or on the endometrium. Aldehyde-modified proteins are highly immunogenic and circulating autoantibodies to new epitopes, such as malondialdehyde (MDA), may affect the reproductive system. Autoantibodies or elevated reactive oxygen species (ROS) in serum are often associated with inflammatory response. The purpose of this work is to investigate whether PCOS women show increased levels of oxidized proteins (protein-MDA) and anti-endometrial antibodies (AEA) in their sera, compared with control patients, and to determine whether AEA specificity is related to oxidized protein derivatives. Sera from 31 women [10 patients with PCOS (PCOS group) and 21 women with male factor of infertility (control group)] were chosen from patients attending for infertility. Anti-endometrial antibodies were determined by enzyme-linked immunosorbent assay (ELISA) with an endometrial cell line (RL-95). Antibodies against MDA modified human serum albumin (HSA-MDA) were also determined by ELISA. Oxidized proteins (protein-MDA) in serum were determined by a colorimetric assay. Patients with PCOS have significantly higher levels of AEA and anti-HSA-MDA, as well as oxidized proteins (protein-MDA) in serum than control patients. For the first time, we describe an autoimmune response in PCOS patients, in terms of AEA. The evidence of protein-MDA in the serum of these patients, together with the increased antibody reactivity to MDA-modified proteins (HSA-MDA) in vitro, supports the conclusion that oxidative stress may be one of the important causes for abnormal endometrial environment with poor embryo receptivity in PCOS patients.