Heterogeneity of engrafted bone-lining cells after systemic and local transplantation
Heterogeneity of engrafted bone-lining cells after systemic and local transplantation
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DOI：
10.1182/blood-2005-02-0582
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发表时间：
2005-11-15
期刊：
影响因子：
20.3
通讯作者：
Rowe, DW
中科院分区：
文献类型：
--
作者：
Wang, LP;Liu, YL;Rowe, DW
使用Col1a1-GFP报告基因转基因小鼠评估各种骨祖细胞移植方案的结果。该模型要求受体小鼠接受致死性全身照射（TBI），然后用全骨髓抢救。当用Col1a1-GFP转基因小鼠的全部骨髓拯救小鼠时，可以在大多数骨内和骨小梁表面观察到绿色荧光蛋白（GFP）阳性的供体细胞。虽然这些细胞表达成骨细胞限制性的绿色荧光蛋白，但它们在体外不能形成矿化基质，也不能形成骨结节。然而，当将同一转基因小鼠的颅骨祖细胞注射到骨髓间隙时，可以观察到供体细胞的成骨作用。使用不同的GFP颜色来区分供体和受体成骨细胞，沿着矿化成骨细胞表面以及在骨内膜骨的骨细胞群内观察到两种细胞类型的混合。尽管注射的祖细胞能够在注射的骨内产生骨，但当外植到原代成骨细胞培养时，它们缺乏形成矿化骨结节的能力。这些试剂和成像方案将有助于评估其他具有比颅骨来源的基质细胞更好的祖细胞潜力的细胞。
The outcome of various osteoprogenitor-cell transplantation protocols was assessed using Col1a1-GFP reporter transgenic mice. The model requires the recipient mice to undergo lethal total body irradiation (TBI) followed by rescue with whole bone marrow. When the mice are rescued with total bone marrow from a Col1a1-GFP transgenic mouse, green fluorescence protein (GFP)-positive donor cells can be observed on most endosteal and trabecular bone surfaces. Although the cells express an osteoblast-restricted GFP, they fall to progress to osteocytes, do not form a mineralized matrix, and do not generate bone nodules in vitro. However when calvarial progenitor cells derived from the same transgenic mice are injected into the bone marrow space, osteogenesis by the donor cells is observed. Using different GFP colors that distinguish the donor and recipient osteoblasts, commingling of the 2 cells types is observed along the mineralizing osteoblast surface as well as within the osteocyte population of the endosteal bone. Despite the ability of the injected progenitor cells to produce bone within the injected bone, they lack the ability to form mineralized bone nodules when explanted to primary osteoblast culture. These reagents and imaging protocols will be useful in evaluating other cells having a better progenitor potential than calvarial-derived stromal cells.