Rapid characterization of plant mutants with an altered ion-profile: a case study using Lotus japonicus
Rapid characterization of plant mutants with an altered ion-profile: a case study using Lotus japonicus
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DOI：
10.1111/j.1469-8137.2008.02730.x
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发表时间：
2009-01-01
期刊：
影响因子：
9.4
通讯作者：
Osaki, Mitsuru
中科院分区：
文献类型：
--
作者：
Chen, Zheng;Watanabe, Toshihiro;Osaki, Mitsuru
豆类对人类的重要性仅次于谷物，研究其营养和其他微量元素的功能基因组学对农业生产和食品强化至关重要。本文描述了一个研究百脉根突变体茎中15种元素积累的离子筛选实验，一个很好的遗传工具，豆类研究。大约2000诱变M2植物培养在一个新的低成本的高通量系统，并通过电感耦合等离子体质谱（ICP-MS）测定其元素概况。通过对M2或M3代植株茎中元素浓度的检测，鉴定出31个元素分布发生改变的突变体。令人惊讶的是，调控必需元素的基因数量与调控非必需元素的基因数量相似。镁（Mg）和镍（Ni）在许多突变体中相关。进一步的研究表明，磷（P）和钴（Co）可能参与了Mg和Ni的离子稳态网络，这些元素的吸收或转运途径与离子转运相关基因密切相关。在本研究中，离子组学被证明是用于确定离子稳态相关基因的强大功能基因组学工具。New Phytologist（2009）181：795- 801 doi：10.1111/j.1469-8137.2008.02730.x。
Legumes are second only to cereals in their importance to humans, and study of their functional genomics of nutrition and other trace elements is crucial for agricultural production and food fortification. We describe here an ionomic screening experiment carried out to investigate the accumulation of 15 elements in shoots of mutants of Lotus japonicus, a good genetic tool for legume study.Approximately 2000 mutagenized M2 plants were cultivated in a novel low-cost high-throughput system and their elemental profiles were determined by inductively coupled plasma mass spectroscopy (ICP-MS).After triple-checking the element concentrations in M2 or M3 plant shoots, 31 mutants with altered elemental profiles were identified. Surprisingly, the number of genes regulating essential elements was similar to the number regulating nonessential elements. Magnesium (Mg) and nickel (Ni) were correlated in a number of mutants. Further investigation suggested that phosphorus (P) and cobalt (Co) might be involved in the ion homeostasis network of Mg and Ni.The results suggested that the pathways for element uptake or translocation were highly linked through the ion transport-related genes. Ionomics proved to be a powerful functional genomics tool for determining genes related to ion homeostasis in this study.New Phytologist (2009) 181: 795-801doi: 10.1111/j.1469-8137.2008.02730.x.