Evaluations for surrounding tissue incorporation after implantation of synthetic vascular prostheses in animal models
Evaluations for surrounding tissue incorporation after implantation of synthetic vascular prostheses in animal models
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DOI：
10.1007/s10047-021-01283-3
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发表时间：
2021-06-18
影响因子：
1.3
通讯作者：
Masumoto, Hidetoshi
中科院分区：
文献类型：
--
作者：
Fujiwara, Yasue;Hirao, Shingo;Masumoto, Hidetoshi
人工血管植入后周围组织的结合可能因植入的假体而异。为了评价植入后组织的结合，我们检查了动物模型植入后的手术、组织学和超微结构结果。测试了三种类型的市售假体（Gelweave（TM）; G组，J Graft SHIELD NEO（R）; J组和Triplex（R）; T组）。将假体植入Sprague-Dawley大鼠皮下或缝合在日本白色家兔腹主动脉上。通过手术检查组织的粘连情况，并通过扫描电子显微镜（SEM）进行细胞和组织浸润的组织学评价和超微结构观察。G组早期粘连形成趋势较低（大鼠：G vs J，P < 0.0001; G vs T，P < 0.0001/兔：G vs J，P < 0.0001; G vs T，P = 0.059）。在晚期，J组显示最高的粘附（大鼠：G vs J，P = 0.0004; J vs T，P = 0.015/兔：G vs J，P = 0.0015; J vs T，P = 0.0044）。在G组中，在植入物和周围组织之间观察到形成包囊的间隙，而其他组在植入物壁内显示组织浸润，这也通过SEM证实。种植体的组织渗透与种植体的粘附呈正相关（P < 0.0001）。周围组织形态根据假体类型而变化。希望阐明每种假体的特征，以便为每个患者选择合适的移植物，以获得更好的手术结果。
Incorporation of surrounding tissues after implantation of synthetic vascular prostheses potentially varies in accordance with implanted prostheses. To evaluate post-implant tissue incorporation, we examined surgical, histological and ultrastructural findings after implantation in animal models. Three types of commercially available prostheses were tested (Gelweave (TM); Group G, J Graft SHIELD NEO(R); Group J and Triplex(R); Group T). Prostheses were implanted into Sprague-Dawley rats subcutaneously or sutured on abdominal aorta of Japanese white rabbits. The tissues were surgically examined for adhesion and were subjected to histological evaluations for cellular and tissue infiltration and ultrastructural observations by scanning electron microscopy (SEM). Group G exhibited less tendency in adhesion formation in early phase (rat: G vs J, P < 0.0001; G vs T, P < 0.0001/rabbit: G vs J, P < 0.0001; G vs T, P = 0.059). In late phase, Group J showed highest adhesion (rat: G vs J, P = 0.0004; J vs T, P = 0.015/rabbit: G vs J, P = 0.0015; J vs T, P = 0.0044). In group G, a gap was observed between implants and surrounding tissues forming capsulation, whereas other groups exhibited tissue infiltration inside of the implants wall which were also confirmed by SEM. The tissue permeation toward the implants and adhesion was positively correlated (P < 0.0001). Surrounding tissue conformation varied in accordance with the type of prostheses. It is desirable to elucidate characteristics of each prosthesis to select suitable grafts for each patient to achieve a better surgical outcome.