Nitrogen removal from digested black water by one-stage partial nitritation and anammox.
Nitrogen removal from digested black water by one-stage partial nitritation and anammox.
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DOI：
10.1021/es803284y
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发表时间：
2009-05
影响因子：
11.4
通讯作者：
S. Vlaeminck;A. Terada;B. Smets;Davy van der Linden;N. Boon;W. Verstraete;M. Carballa
中科院分区：
文献类型：
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作者：
S. Vlaeminck;A. Terada;B. Smets;Davy van der Linden;N. Boon;W. Verstraete;M. Carballa
本研究评估了在实验室规模的限氧自养硝化/反硝化（OLAND）旋转生物接触器中处理来自真空厕所（> 1000 mg NH 4（+）-N L（-1））的消化黑水的技术可行性。经过2.5个月的适应期后，脱氮率稳定在约100%。在随后的5个月内达到700 mg N L（-1）d（-1）。当游离氨浓度高于3 mg N L（-1）时，亚硝酸盐氧化菌的抑制作用可使脱氮效率达到76%。适宜的有机碳氮比和无机碳氮比分别保证了厌氧氨氧化活性和足够的缓冲能力。定量荧光原位杂交结果表明，好氧和缺氧铵氧化细菌（AerAOB和AnAOB）分别占生物膜的43%和8%。由于部分AerAOB可能存在于缺氧生物膜区，因此它们的比铵转化率非常低，与高比AnAOB活性相反。DGGE分析表明，优势种AerAOB和AnAOB对从合成培养基到消化黑水的过渡具有抗性。本研究表明，高速率脱氮消化黑水一阶段部分亚硝化和厌氧氨氧化，这将允许显着降低运营成本相比，传统的硝化/反硝化。
This study assessed the technical feasibility to treat digested black water from vacuum toilets (> 1000 mg NH4(+)-N L(-1)) in a lab-scale oxygen-limited autotrophic nitrification/denitrification (OLAND) rotating biological contactor. After an adaptation period of 2.5 months, a stable nitrogen removal rate of ca. 700 mg N L(-1) d(-1) was reached over the subsequent 5 months. Suppression of the nitrite oxidizing bacteria at free ammonia levels above 3 mg N L(-1) resulted in a nitrogen removal efficiency of 76%. The favorable ratios of both organic and inorganic carbon to nitrogen guaranteed endured anammox activity and sufficient buffering capacity, respectively. Quantitative FISH showed that aerobic and anoxic ammonium-oxidizing bacteria (AerAOB and AnAOB) made up 43 and 8% of the biofilm, respectively. Since a part of the AerAOB was probably present in anoxic biofilm zones; their specific ammonium conversion was very low, in contrast to the high specific AnAOB activity. DGGE analysis showed that the dominant AerAOB and AnAOB species were resistant to the transition from synthetic medium to digested black water. This study demonstrates high-rate nitrogen removal from digested black water by one-stage partial nitritation and anammox, which will allow a significant decrease in operational costs compared to conventional nitrification/ denitrification.