Improvement of foam breaking and oxygen-transfer performance in a stirred-tank fermenter
Improvement of foam breaking and oxygen-transfer performance in a stirred-tank fermenter
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DOI：
10.1007/s00449-005-0028-x
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发表时间：
2006-03
影响因子：
3.8
通讯作者：
Satoshi Takesono;M. Onodera;K. Toda;M. Yoshida;K. Yamagiwa;A. Ohkawa
中科院分区：
文献类型：
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作者：
Satoshi Takesono;M. Onodera;K. Toda;M. Yoshida;K. Yamagiwa;A. Ohkawa
本研究探讨了搅拌槽发酵罐（STF）含有低粘度发泡液体与搅拌叶轮和泡沫破碎叶轮安装在同一轴上。结果表明，将传统的六叶涡轮机桨改为棒状桨作为搅拌桨，可以改善泡沫破碎桨的性能。采用六叶片叶片圆盘作为杆式搅拌桨的机械泡沫控制方法（MFM）的体积氧传递系数kLa约为采用六叶片涡轮机搅拌桨的化学泡沫控制方法（CFM）的5倍。应用本方法培养Saccharomycescleae K-7表明，达到最大细胞浓度的培养时间明显短于使用常规CFM实现的培养时间。
This study examined a stirred-tank fermenter (STF) containing low-viscosity foaming liquids with an agitation impeller and foam-breaking impeller mounted on the same shaft. Results showed that the performance of the foam-breaking impeller can be improved by changing a conventional six-blade turbine impeller into a rod impeller as the agitation impeller. The volumetric oxygen-transfer coefficient,kLa, in the mechanical foam-control method (MFM) using a six-blade vaned disk as the foam-breaking impeller in the STF with the rod impeller was approximately five times greater than that of the chemical foam-control method (CFM) adding an anti-foaming agent in the STF with the six-blade turbine impeller. Application of the present method to the cultivation ofSaccharomyces cerevisiaeK-7 demonstrated that the cultivation time up to the maximum cell concentration was remarkably shorter than that achieved using a conventional CFM.