Chemical modification of lipase with a comb-shaped synthetic copolymer of polyoxyethylene allyl methyl diether and maleic anhydride

Chemical modification of lipase with a comb-shaped synthetic copolymer of polyoxyethylene allyl methyl diether and maleic anhydride
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聚氧乙烯烯丙基甲基二醚与马来酸酐梳状合成共聚物对脂肪酶的化学修饰

DOI:
10.1007/bf01023940
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发表时间:
1992
影响因子:
2.7
通讯作者:
Y. Inada
Y. Inada
中科院分区:
工程技术4区
文献类型:
--
作者:
M. Hiroto;A. Matsushima;Y. Kodera;Yasutsugu Shibata;Y. Inada

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荧光假单胞菌的脂肪酶与聚氧乙烯基烯丙基甲基二醚和顺丁烯二酸酐的共聚物偶联,活化PMPM-脂肪酶在有机溶剂中具有较强的溶解性和活性,且具有较好的热稳定性。它在苯中催化酯的合成,在水相体系中催化酯的水解,具有较高的酶活性。
SummaryLipase fromPseudomonas fluorescens was coupled with a copolymer of polyoxyethylene allyl methyl diether and maleic anhydride, activated PM. The PM-lipase became soluble and active in organic solvents, and also heat stable. It catalyzed the ester synthesis in benzene and ester hydrolysis in an aqueous system with high enzymic activity.
A.Nakajima、T.Hayashi、H.Sato:“多相生物医学材料”VSP,荷兰乌得勒支,21-40 (1989)
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