Demonstration of Low-Regulatory CD25High+CD4+ and High-Pro-inflammatory CD28−CD4+ T-Cell Subsets in Patients with Ulcerative Colitis: Modified by Selective Granulocyte and Monocyte Adsorption Apheresis
Demonstration of Low-Regulatory CD25High+CD4+ and High-Pro-inflammatory CD28−CD4+ T-Cell Subsets in Patients with Ulcerative Colitis: Modified by Selective Granulocyte and Monocyte Adsorption Apheresis
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DOI：
10.1007/s10620-006-9560-z
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发表时间：
2007-04
影响因子：
3.1
通讯作者：
Y. Yokoyama;K. Fukunaga;Y. Fukuda;Katsuyuki Tozawa;K. Kamikozuru;K. Ohnishi;Takeshi Kusaka;T. Kosaka;N. Hida;Y. Ohda;H. Miwa;T. Matsumoto
中科院分区：
文献类型：
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作者：
Y. Yokoyama;K. Fukunaga;Y. Fukuda;Katsuyuki Tozawa;K. Kamikozuru;K. Ohnishi;Takeshi Kusaka;T. Kosaka;N. Hida;Y. Ohda;H. Miwa;T. Matsumoto
低CD25High+−+、调节性CD25+CD4+T细胞亚群和高炎症CD28High+CD4+T细胞可加重溃疡性结肠炎(UC)。本研究旨在探讨UC患者外周血中CD25High+CD_4~+T细胞和CD_(28)−CD_4~+T细胞的频率及其在粒细胞和单核细胞吸附分离术中的变化。受试者为活动期UC患者12例，静止期UC患者11例，健康志愿者14例。活动期UC患者的平均临床活动指数为15.7±0.22，静止期UC患者的平均临床活动指数为4.5±0.11。外周血标本进行CD4、CD25和CD28抗体染色，进行流式细胞仪检测。活动期UC患者接受GMA治疗，并在第一次GMA治疗前后检测血样。活动期UC(P<；0.04)和静止期UC(P<；0.02)患者的CD28CD4+T细胞百分比均高于HV(P=0.03)，而CD28+−+T细胞百分比均低于HV(P=0.03和P<；0.02)。活动期UC患者CD25High+CD_4~+T细胞比例低于静止期UC患者(P<；0.001)。CD25High+CD4+T细胞的显著增加与GMA有关(P<；0.03)。低CD25High+−+和高CD28High+CD4+是UC的显著特征。GMA诱导的CD25High+CD4+T细胞的增加可能有助于免疫功能的改善。由于CD25High+CD_4+−和CD_(28)−CD_4+−表达T细胞的频率较高可能是临床疗效的预测因素，因此有必要进行进一步的研究。
Low-CD25High+CD4+, a subset of regulatory CD25+CD4+T cells and high-inflammatory CD28−CD4+T cells can exacerbate ulcerative colitis (UC). This study sought to investigate the frequency of CD25High+CD4+and CD28−CD4+T cells in patients with UC and the changes in these cells during Adacolumn granulocyte and monocyte adsorption apheresis (GMA). Subjects were 12 patients with active UC, 11 with quiescent UC, and 14 healthy volunteers (HVs). The mean clinical activity index was 15.7 ± 2.2 in active UC and 4.5 ± 1.1 in quiescent UC. Peripheral blood samples were stained with CD4, CD25, and CD28 antibodies for flow cytometry. Patients with active UC received GMA and blood samples were examined before and after the first GMA session. Patients with active UC (P< 0.04) or quiescent UC (P< 0.02) had a higher percentage of CD28−D4+T cells compared with HVs, while the percentage of CD28+CD4+T cells was lower in both UC groups compared with HVs (P= 0.03 andP< 0.02). Patients with active UC had a lower percentage of CD25High+CD4+T cells compared with quiescent UC patients (P< 0.001). A significant increase in CD25High+CD4+T cells was associated with GMA (P< 0.03). Low CD25High+CD4+and high CD28−CD4+are prominent features in UC. The increase in CD25High+CD4+T cells induced by GMA should contribute to improved immune function. Additional studies are warranted, since a low frequency of CD25High+CD4+−and a high frequency of CD28−CD4+−expressing T cells might be a predictor of clinical response to GMA.