Isolation of a MADS-box gene (ERAF17) and correlation of its expression with the induction of formation of female flowers by ethylene in cucumber plants (Cucumis sativus L.)
Isolation of a MADS-box gene (ERAF17) and correlation of its expression with the induction of formation of female flowers by ethylene in cucumber plants (Cucumis sativus L.)
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DOI：
10.1007/s004250100571
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发表时间：
2001-10-01
期刊：
影响因子：
4.3
通讯作者：
Sakai, S
中科院分区：
文献类型：
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作者：
Ando, S;Sato, Y;Sakai, S
乙烯对黄瓜性别表达的调控植物当雌雄同株黄瓜幼苗（cv.用乙烯利处理下白（Shimoshirazu-jibai），在节上诱导雌花。为了阐明乙烯在性别表达调控中的作用，我们试图分离乙烯利处理后，这些黄瓜植株顶端雌花形成诱导过程中表达发生变化的基因。使用差异显示方法，我们确定了20个克隆（#1至#20），反映了用乙烯利处理或未处理的顶端中转录物积累的差异。cDNA片段的序列分析显示CDNA#17具有MADS盒基因的序列。我们分离了全长cDNA，并表明它包括一个MAIDS盒和一个K盒，相应的基因被命名为ERAF 17。我们检测了ERAF 17在cv.下白（Shimoshirazu-jibai）和雌株栽培品种（Rensei）中的那些。在这些品种中，ERAF 17转录本的表达时间和水平与雌花的发育相关。乙烯利诱导ERAF 17转录本的合成发生在处理开始后4小时内，并至少持续4天。ERAF 17在顶端的表达定位于雌蕊品种的花芽中，并且在雌花的整个发育过程中保持表达。我们的研究结果表明，乙烯诱导雌花的形成可能是通过ERAF 17在黄瓜植株顶端的花芽中的表达来调节的，并且该基因的表达也可能参与雌花的发育。
Ethylene regulates sex expression in cucumber (Cucumis sativus L.) plants. When the apices of monoecious cucumber seedlings (cv. Shimoshirazu-jibai) were treated with the ethylene-releasing compound, ethephon, female flowers were induced at the nodes. To clarify the action of ethylene in the regulation of sex expression, we attempted to isolate genes whose expression changed during induction of the formation of female flowers at the apices of these cucumber plants upon treatment with ethephon. Using the differential-display method, we identified 20 clones (#1 to #20) that reflected differences in the accumulation of transcripts in apices treated or not treated with ethephon. Sequence analysis of cDNA fragments revealed that the cDNA #17 had the sequence of a MADS-box gene. We isolated the full-length cDNA and showed that it included both a MAIDS box and a K box, and the corresponding gene was designated ERAF17. We examined the expression of ERAF17 in the apices of cv. Shimoshirazu-jibai and in those of a gynoecious cultivar (Rensei). In these cultivars, the timing and levels of expression of the ERAF17 transcript were correlated with the development of female flowers. Induction of the synthesis of the ERAF17 transcript by ethephon occurred within 4 h of the start of treatment and continued for 4 days at least. Expression of ERAF17 at apices was localized in the floral buds of the gynoecious cultivar, and expression was maintained in female flowers thorough their development. Our results suggest that the induction of the formation of female flowers by ethylene might be regulated by the expression of ERAF17 in floral buds at the apices of cucumber plants and that expression of this gene might also be involved in the development of female flowers.