Efficacy of 50 Hz Electromagnetic Fields on Human Epidermal Stem Cell Transplantation Seeded in Collagen Sponge Scaffolds for Wound Healing in a Murine Model
Efficacy of 50 Hz Electromagnetic Fields on Human Epidermal Stem Cell Transplantation Seeded in Collagen Sponge Scaffolds for Wound Healing in a Murine Model
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50 Hz 电磁场对接种在胶原海绵支架中的人类表皮干细胞移植用于小鼠模型伤口愈合的功效
DOI：
10.1002/bem.22029
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发表时间：
2017-04-01
影响因子：
1.9
通讯作者：
Zhang, Ming-Sheng
中科院分区：
文献类型：
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作者：
Bai, Wen-Fang;Xu, Wei-Cheng;Zhang, Ming-Sheng
为探讨电磁场（EMF）在皮肤组织工程中的可能功效，在小鼠模型中研究了EMF暴露对表皮干细胞（ESC）在胶原海绵支架上的伤口愈合的影响。将36只7 ~ 8周龄裸鼠建立的全层缺损创面模型随机分为3组：对照组、单纯ESC组和ESC + EMF暴露组（频率50 Hz，磁感应强度5 mT，60 min/d，持续20 d）。从人包皮中分离ESC并进行体外培养，然后将胶原海绵支架作为递送载体移植到仅ESC组的伤口处，ESC + EMF暴露组在ESC移植后暴露于EMF。观察电磁场对再生皮肤形态学变化及β1整合素表达的影响。观察创面愈合率和愈合时间，评价修复效果。结果表明，人胚胎干细胞成功地移植到裸鼠体内，促进了裸鼠完整皮肤的形成。与其他组相比，ESCs + EMF组创面愈合最快（P < 0.05），再生皮肤结构更成熟，活细胞层数更连续，毛囊结构更丰富。这些结果表明，使用50 Hz EMF作为非侵入性治疗可以加速ESC移植的伤口愈合，并恢复再生皮肤的结构完整性。生物电磁学38：204- 212，2017。© 2017 Wiley Periodicals，Inc.
To explore the possible efficacy of electromagnetic fields (EMF) for skin tissue engineering, effects of EMF exposure on epidermal stem cells (ESC) seeded in collagen sponge scaffolds for wound healing in a murine model were investigated. The wound models of a full‐thickness defect established with 36 7 ∼ 8‐week‐old nude mice were randomly divided into three groups: a control group, an ESC‐only group, and an ESC with EMF exposure group (frequency of 50 Hz, magnetic induction of 5 mT, 60 min per day for 20 days). ESC were separated from human foreskin and cultured in vitro, and then transplanted with collagen sponge scaffolds as a delivery vehicle to wounds of the ESC‐only group, and ESC with EMF exposure group was exposed to EMF after ESC transplantation. Effects of EMF on morphological changes and expression of β1 integrin in regenerated skins were observed. Wound healing rates and healing times were collected to evaluate the efficacy of repairment. Results showed that human ESC were successfully transplanted to nude mice, which facilitated the formation of intact skin on nude mice. In contrast to other groups, the wound healing of ESC with EMF exposure group was the fastest (P < 0.05), the structure of regenerated skins was more mature, and it contained more continuity in the number of viable cell layers and rich hair follicles’ structure. These results suggest that the use of 50 Hz EMF as a non‐invasive treatment can accelerate wound healing of ESC transplantation, and restore structural integrity of regenerated skin. Bioelectromagnetics. 38:204–212,2017. © 2017 Wiley Periodicals, Inc.