Oxytocin prevents dysregulation of the acute stress response and glucocorticoid-induced oxidative stress in chronically isolated prairie voles.
Oxytocin prevents dysregulation of the acute stress response and glucocorticoid-induced oxidative stress in chronically isolated prairie voles.
复制标题
催产素可防止长期隔离的草原田鼠急性应激反应和糖皮质激素诱导的氧化应激的失调。
DOI：
10.1016/j.psyneuen.2023.106121
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发表时间：
2023
影响因子：
3.7
通讯作者：
Haussmann,MarkF
中科院分区：
文献类型：
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作者：
Stevenson,JennieR;McMahon,ElyseK;McNeely,TaylorL;Haussmann,MarkF
长期的社会隔离会导致许多生理和心理过程的失调，包括对急性压力源的反应能力。我们实验室以前的工作报告说，草原田鼠（Microtus ochrogaster）六周的社会隔离导致糖皮质激素水平增加，氧化损伤，端粒降解和快感缺乏，催产素治疗阻止了所有这些变化。根据这些结果，我们研究了慢性社会隔离与催产素治疗和不影响糖皮质激素（CORT）和氧化应激反应的急性应激源，5分钟的居民入侵者（R-I）测试结束时的社会隔离期。为了研究短暂的急性应激对CORT和氧化应激的影响，在R-I测试前24小时，在社会隔离6周后收集基线血液样本。在R-I试验结束后15分钟再采集两份血样，并在25分钟后再次采集血样，分别测量峰值和恢复响应。与未经历隔离的动物相比，隔离动物具有更高的CORT和活性氧代谢物（ROM，氧化应激的测量）的基线、峰值、恢复和综合水平。重要的是，在整个隔离期间，催产素治疗防止了CORT和ROM的这些升高。总抗氧化能力（TAC）没有显着变化。峰值和恢复时间点的CORT和ROM水平呈正相关。这些数据表明，急性应激在慢性隔离草原田鼠，然后，与糖皮质激素诱导的氧化应激（GiOS）增加，催产素减轻隔离诱导的糖皮质激素和氧化应激急性应激反应失调。
Chronic social isolation can lead to dysregulation of many physiological and psychological processes, including the ability to respond to acute stressors. Previous work in our lab reported that six weeks of social isolation in prairie voles (Microtus ochrogaster)caused increased glucocorticoid levels, oxidative damage, telomere degradation and anhedonia, and that oxytocin treatment prevented all of these changes. Following these results, we investigated how chronic social isolation with and without oxytocin treatment affected glucocorticoid (CORT) and oxidative stress responses to an acute stressor, a 5-min resident-intruder (R-I) test at the end of the social isolation period. To investigate the effect of a brief acute stressor on CORT and oxidative stress, baseline blood samples were collected following six weeks of social isolation, 24-hrs before the R-I test. Two more blood samples were collected 15-min after the end of the R-I test, and again 25-min later to measure peak and recovery responses, respectively. Isolated animals had higher baseline, peak, recovery, and integrated levels of CORT and reactive oxygen metabolites (ROMs, a measure of oxidative stress), compared to animals that did not experience isolation. Importantly, oxytocin treatment throughout the isolation period prevented these elevations in CORT and ROMs. No significant changes were observed in total antioxidant capacity (TAC). Levels of CORT and ROMs at the peak and recovery time points were positively correlated. These data show that acute stress in chronically isolated prairie voles, then, is associated with increased glucocorticoid-induced oxidative stress (GiOS), and that oxytocin mitigates isolation-induced dysregulation of glucocorticoid and oxidative stress acute stress responses.