Saccharide polymer brushes to control protein and cell adhesion to titanium.
Saccharide polymer brushes to control protein and cell adhesion to titanium.
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DOI：
10.1021/bm8011924
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发表时间：
2009-03
影响因子：
6.2
通讯作者：
J. E. Raynor;T. Petrie;K. Fears;R. Latour;Andrés J. García;D. Collard
中科院分区：
文献类型：
--
作者：
J. E. Raynor;T. Petrie;K. Fears;R. Latour;Andrés J. García;D. Collard
控制钛的表面化学对其在医疗植入物中的应用至关重要，特别是在解决感染和假体松动等并发症方面，随着时间的推移，这些并发症仍然存在巨大的挑战。表面引发的原子转移自由基聚合(SI-ATRP)是一种糖基取代的甲基丙烯酸-2-葡萄糖酰胺基甲基丙烯酸乙酯(GAMA)，它提供了致密的聚合物刷子，可以抵抗蛋白质吸附和细胞黏附。我们进一步通过粘附肽的共价连接来定制表面的性质，以提供对细胞粘附性的控制。未经修饰的聚(GAMA)刷子可防止细胞黏附，而含有GFOGER多肽序列的刷子可促进融合均匀分布的细胞的沉积。在强健的生物抗性背景上以这种方式呈现黏附蛋白构成了开发新生物材料的一种通用方法。
Attaining control over the surface chemistry of titanium is critical to its use in medical implants, especially to address complications such as infection and loosening of implants over time, which still present significant challenges. The surface-initiated atom transfer radical polymerization (SI-ATRP) of a saccharide-substituted methacrylate, 2-gluconamidoethyl methacrylate (GAMA), affords dense polymer brushes that resist protein adsorption and cell adhesion. We further tailored the nature of the surfaces by covalent attachment of an adhesion peptide to afford control over cell adhesion. Whereas unmodified poly(GAMA) brushes prevent cell adhesion, brushes with a tethered GFOGER-containing peptide sequence promote the deposition of confluent well-spread cells. The presentation of adhesion proteins on a robust bioresistive background in this fashion constitutes a versatile approach to the development of new biomaterials.