Cytogenetic diversity of Korean hexaploid wheat (Triticum aestivum L.) with simple sequence repeats (SSRs) by fluorescence in situ hybridization.
Cytogenetic diversity of Korean hexaploid wheat (Triticum aestivum L.) with simple sequence repeats (SSRs) by fluorescence in situ hybridization.
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DOI：
10.1007/s12892-018-0245-0
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发表时间：
2018-01-01
影响因子：
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通讯作者：
Kang, C. S.
中科院分区：
文献类型：
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作者：
Cho, Seong-Woo;Kang, Seong-Wook;Kang, C. S.
对朝鲜六倍体小麦的染色体进行了研究，比较了染色体核型的细胞遗传多样性。用四甲基罗丹明5-dUTP和荧光素12-dUPT分别标记两种类型的简单重复序列（SSR）（AAG）5和（AAC）5进行原位杂交，进行染色体核型分析。这两个SSR标记的细胞遗传学特征表明，小麦染色体的细胞遗传学特征明显为B染色体组。在这项研究中，朝鲜六倍体小麦品种金康的染色体核型是A、B和D基因组，用作细胞遗传学参考。这两个SSR标记的表达信号表明，韩国六倍体小麦1B染色体核型存在差异。（AAG）5和（AAC）5信号在1B染色体短臂上的分布模式和浓缩程度在韩国六倍体小麦中不同，从高到低依次为：Keumkang > Joeun > Johan > Olgeuru。Olgeuru与其他韩国六倍体小麦相比，两个SSR信号的分布和浓缩水平较低。在A染色体组中，在几个韩国六倍体小麦中，7A染色体在短臂和长臂的远端区域上显示出不平衡的（AAG）5信号表达，而韩国六倍体小麦Joeun在7A染色体的每个臂的远端区域上显示出明确的（AAG）5信号。在韩国六倍体小麦中，与其他韩国六倍体小麦相比，韩国六倍体糯小麦Shinmichal 1具有具有（AAG）5和（AAC）5独特表达模式的染色体。这些细胞遗传学的差异，在这项研究中确定是有用的指标，以提高韩国小麦育种计划的细胞遗传多样性。
Chromosomes of Korean hexaploid wheat were investigated to compare the chromosomal karyotype for cytogenetic diversity. Chromosomal karyotyping was done with in situ hybridization using two types of simple sequence repeats (SSR)s, (AAG)5 and (AAC)5 labeled with tetramethyl-rhodamine-5-dUTP and fluorescein-12-dUPT as a fluorescence, respectively. The two SSRs as cytogenetic markers revealed that the cytogenetic characteristics of the wheat chromosomes were remarkably a B genome. In this study, the chromosomal karyotype of Keumkang, a Korean hexaploid wheat cultivar, was the A, B, and D genomes used as a cytogenetic reference. The expressed signals from the two SSRs showed a difference in the chromosomal karyotype of chromosome 1B among the Korean hexaploid wheat. The distribution pattern and the degree of condensation for the (AAG)5 and (AAC)5 signals on the short arm of chromosome 1B were different in the Korean hexaploid wheat shown in descending order: Keumkang > Joeun > Johan > Olgeuru. Olgeuru had a lower level of distribution and condensation for the two SSRs signals compared to the other Korean hexaploid wheat. In the A genome, chromosome 7A showed an unbalanced expression of the (AAG)5 signal on the distal region of the short and long arms in several Korean hexaploid wheat while Joeun, a Korean hexaploid wheat, showed a definite (AAG)5 signal on the distal region of each arm of chromosome 7A. Among the Korean hexaploid wheat, Shinmichal1, a Korean hexaploid waxy wheat, had a chromosome with a unique expression pattern for (AAG)5 and (AAC)5 compared the other Korean hexaploid wheat. Those cytogenetic differences identified in this study are useful as an indicator to improve the cytogenetic diversity in the Korean wheat breeding program.