Cloning and characterization of SPL-family genes in the peanut (Arachis hypogaea L.).
Cloning and characterization of SPL-family genes in the peanut (Arachis hypogaea L.).
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DOI：
10.4238/gmr.15017344
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发表时间：
2016-02
期刊：
影响因子：
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通讯作者：
M. Li;S. Z. Zhao;C. Zhao;Y. Zhang;H. Xia;J. López-Baltazar;S. Wan;X. Wang
中科院分区：
文献类型：
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作者：
M. Li;S. Z. Zhao;C. Zhao;Y. Zhang;H. Xia;J. López-Baltazar;S. Wan;X. Wang
SQUAMOSA 启动子结合蛋白样 (SPL) 蛋白在植物生长、发育和对环境应激的反应中发挥着至关重要的作用。花生（ArachishypogaeaL.）是一种全球重要的油料作物。本研究通过转录组测序和cDNA末端快速扩增技术克隆了花生中15个SPL的全长cDNA，并分析了其基因组DNA序列。 cDNA 长度差异显着，从 369 bp 到 3102 bp。与其他植物物种的 SPL 相比，花生 SPL 蛋白的 SBP 结构域高度保守。根据花生 SPL 与其他植物物种的 SPL 的序列相似性，可以将花生 SPL 分为五个亚组。在每个亚组中，单个基因的长度、保守基序数量和分布模式相似。其中 7 个 SPL 被预测为 miR156 的靶标。 SPL在根、叶、花、雌蕊和种子中普遍表达，具有不同的表达水平和积累模式。在幼叶和成年叶之间观察到大多数 SPL 表达的显着差异，表明它们参与发育调节。在第 1 阶段（空中生长的绿色雌蕊）、第 2 阶段（雌蕊在土壤中埋藏约三天）和第 3 阶段（雌蕊在土壤中埋藏约 9 天，豆荚增大），转录物水平发生动态变化。讨论了这些基因在花生荚形成中可能发挥的作用。
SQUAMOSA promoter-binding protein-like (SPL) proteins play crucial roles in plant growth, development, and responses to environmental stressors. The peanut (Arachis hypogaea L.) is a globally important oil crop. In this study, we cloned the full-length cDNA of 15 SPLs in the peanut by transcriptome sequencing and rapid amplification of cDNA ends, and analyzed their genomic DNA sequences. cDNA lengths varied significantly, from 369 to 3102 bp. The SBP domain of the peanut SPL proteins was highly conserved compared to SPLs in other plant species. Based on their sequence similarity to SPLs from other plant species, the peanut SPLs could be grouped into five subgroups. In each subgroup, lengths of individual genes, conserved motif numbers, and distribution patterns were similar. Seven of the SPLs were predicted to be targets of miR156. The SPLs were ubiquitously expressed in the roots, leaves, flowers, gynophores, and seeds, with different expression levels and accumulation patterns. Significant differences in the expression of most of the SPLs were observed between juvenile and adult leaves, suggesting that they are involved in developmental regulation. Dynamic changes occurred in transcript levels at stage 1 (aerial grown green gynophores), stage 2 (gynophores buried in soil for about three days), and stage 3 (gynophores buried in soil for about nine days with enlarged pods). Possible roles that these genes play in peanut pod initiation are discussed.