MYOBLASTS TRANSFERRED TO THE LIMBS OF EMBRYOS ARE COMMITTED TO SPECIFIC FIBER FATES
MYOBLASTS TRANSFERRED TO THE LIMBS OF EMBRYOS ARE COMMITTED TO SPECIFIC FIBER FATES
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DOI：
10.1038/362165a0
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发表时间：
1993-03-11
期刊：
影响因子：
64.8
通讯作者：
STOCKDALE, FE
中科院分区：
文献类型：
--
作者：
DIMARIO, JX;FERNYAK, SE;STOCKDALE, FE
在肢芽的5天大的禽胚胎，当初级肌纤维形成开始和特定的肌肉出现之前，快和慢肌球蛋白重链基因的表达差异，可以检测到初级纤维的前肌质量1，2。可以从这些肢芽中分离出形成特定类型纤维集落的成肌细胞3。为了评估成肌细胞承诺在胚胎发育过程中指定纤维类型的作用，我们从胚胎和成人肌肉中克隆了特定类型的成肌细胞，用报告基因转染它们，并将它们转移到发育中的肢芽中。移植后，克隆的成肌细胞在肢体中形成纤维，其肌球蛋白重链基因表达模式与它们在细胞培养中形成的纤维相同。这些结果表明，初始骨骼肌纤维类型的多样性在禽肢体发育过程中可以起源，部分，从不同的成肌细胞类型的承诺，形成特定的纤维类型。
IN the limb bud of the 5-day-old avian embryo, when primary muscle fibre formation is beginning and before specific muscles appear, differences in the expression of fast and slow myosin heavy chain genes can be detected among primary fibres of the premuscle masses1,2. Myoblasts that form colonies of fibres of specific types can be isolated from these limb buds3. To assess the role of myoblast commitment in specifying fibre types during embryonic development, we cloned myoblasts of specific types from embryonic and adult muscles, transfected them with a reporter gene, and transferred them into developing limb buds. After transfer, cloned myoblasts formed fibres in the limb with the same patterns of myosin heavy chain gene expression as the fibres they formed in cell culture. These results demonstrate that initial skeletal muscle fibre type diversity during avian limb development can originate, in part, from the commitment of distinct myoblast types to the formation of specific fibre types.