Increasing bacterial cellulose compression resilience with glycerol or PEG400 for robuster engineered living materials.
Increasing bacterial cellulose compression resilience with glycerol or PEG400 for robuster engineered living materials.
复制标题
使用甘油或 PEG400 提高细菌纤维素的压缩弹性，以获得更坚固的工程活性材料。
DOI：
10.1016/j.carpta.2022.100245
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发表时间：
2022
影响因子：
5.5
通讯作者：
Caro-Astorga J
中科院分区：
文献类型：
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作者：
Caro-Astorga J
细菌纤维素（BC）是目前处于工程生命材料（ELMs）研究创新边缘的天然材料之一，因为其易于生长和作为水凝胶的优异性能。然而，这种材料的主要限制之一是，当水分子离开多孔网络时，它在开放环境中快速脱水。在这里，我们表明，其他溶剂具有较高的蒸发温度，即甘油和聚乙二醇（PEG），可以发挥相同的作用，水内的BC结构与纤维素纤维通过氢键相互作用。我们证明，这些分子以浓度依赖的方式使BC水凝胶的杨氏模量提高了130倍。为了利用这些效果应用于由Komagataeibacter rhaeticus产生的基于BC的ELMs，我们还探索了甘油和PEG 400对BC片中BC产生细菌的存活的影响。20%的PEG 400使材料对压缩力的弹性增加了一倍，仍然允许细菌在材料中存活数周。这些结果为探索新的应用和堆叠存储条件提供了进一步的机会。
Bacterial cellulose (BC) is one of the current natural materials at the edge of innovation in engineered living materials (ELMs) research due to its ease of growth and outstanding properties as a hydrogel. One of the main limitations of this material, however, is its quick dehydration in open environments as water molecules leave the porous network. Here we show that other solvents with higher evaporation temperatures, namely glycerol and polyethylene glycol (PEG), can play the same role as water within the BC structure interacting with cellulose fibres via hydrogen bonds. We demonstrate that these molecules provide up to a 130-fold improvement in the Young´s Modulus of BC hydrogels to compression forces in a concentration dependent manner. To take advantage of these effects for application in BC-based ELMs produced byKomagataeibacter rhaeticus, we also explored the effect of glycerol and PEG400 on the survival of the BC-producing bacteria in BC pieces. PEG400 at 20% doubled the material resilience to compression forces, still allowing bacteria to survive within the material for weeks. These results open further opportunities to explore new applications and stacked storage conditions.