Near-Complete Structural Characterization of Phosphatidylcholines Using Electron Impact Excitation of Ions from Organics
Near-Complete Structural Characterization of Phosphatidylcholines Using Electron Impact Excitation of Ions from Organics
复制标题
DOI：
10.1021/acs.analchem.5b01460
复制
发表时间：
2015-06-02
影响因子：
7.4
通讯作者：
Baba, Takashi
中科院分区：
文献类型：
--
作者：
Campbell, J. Larry;Baba, Takashi
虽然脂质是许多细胞、组装和生物途径的关键组分，但其分子结构的准确描述仍然难以获得。许多台式表征方法需要费力和十分消耗的程序，并且每当探测新的结构特征时都需要多次测定。在这里;我们描述了一种用于增强结构脂质组学的新的基于质谱的工作流程，其在单个实验中可以产生给定甘油磷脂（GPL）种类的几乎完整的结构信息。这包括来自完整脂质离子和特征头基片段的精确质量的脂质总和（Brutto）组成、来自sn-1和位置特有的片段离子的区域异构体组成以及脂质酰基链中碳-碳双键的位置。来自有机物的离子的电子碰撞激发（EIEIO）-一种技术，其中单电荷脂质离子被电子束照射，产生诊断产物离子。我们已经评估了这种方法的各种脂质标准品，以及生物提取物，以证明这种新方法的实用性。
Although lipids are critical components, of many cellular, assemblies and biological pathways, accurate descriptions of their molecular structures remain difficult to obtain. Many benchtop characterization methods require arduous and tithe-consuming procedures, and multiple assays are required whenever a new structural feature is probed. Here; we describe a new mass-spectrometry-based workflow for enhanced structural lipidomics that, in a single experiment, can yield almost complete structural information for a given glycerophospholipid (GPL) species. This includes, the lipid's sum (Brutto) composition from the accurate mass measured for the intact lipid ion and the characteristic headgroup fragment, the regioisomer composition from fragment ions unique to the sn-1 and positions, and the positions of carbon-carbon double bonds in the lipid acyl chains Here, lipid ions are fragmented using, electron impact excitation of ions from organics (EIEIO)-a technique where the singly charged lipid ions are irradiated by an electron beam, producing diagnostic product ions. We have evaluated this methodology on various lipid standards, as well as on a biological extract, to demonstrate this new method's utility.