Sorghum mutant RG displays antithetic leaf shoot lignin accumulation resulting in improved stem saccharification properties
Sorghum mutant RG displays antithetic leaf shoot lignin accumulation resulting in improved stem saccharification properties
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高粱突变体 RG 表现出相反的叶芽木质素积累，从而改善茎糖化特性
DOI：
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发表时间：
2013
影响因子：
6.3
通讯作者：
Seth Debolt
中科院分区：
文献类型：
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作者：
Carloalberto Petti;A. Harman;Mizuki Tateno;Rekha Kushwaha;A. Shearer;A. Downie;M. Crocker;Seth Debolt
背景提高生物能源作物品种的糖化效率仍然是一个重要的挑战。在这里，我们报告的特性Sorcerebicolor L.结果发现RG表现出与野生型相反的叶片木质素积累增加和茎木质素消耗减少的趋势。与这些测量一致，RG叶组织显示出降低的糖化效率，而茎糖化效率相对于野生型增加。减少的木质素与RG茎中的糖化作用改善有关，但也观察到RG茎木质素中S：G比更大的化学位移。在纤维素含量和结构的相似性，通过XRD分析支持糖化性能的增加和减少木质素，而不是在纤维素的组成和/或structure.ConclusionAntithetic木质素积累的变化之间的相关性观察RG突变体叶和茎组织，这导致更大的糖化效率在RG茎和差分热化学产品产量在高木质素叶。因此，RG突变体的红叶着色代表了C4草高粱中茎纤维素转化为可发酵糖的改善的潜在标记。
BackgroundImproving saccharification efficiency in bioenergy crop species remains an important challenge. Here, we report the characterization of a Sorghum (Sorghum bicolor L.) mutant, named REDforGREEN (RG), as a bioenergy feedstock.ResultsIt was found that RG displayed increased accumulation of lignin in leaves and depletion in the stems, antithetic to the trend observed in wild type. Consistent with these measurements, the RG leaf tissue displayed reduced saccharification efficiency whereas the stem saccharification efficiency increased relative to wild type. Reduced lignin was linked to improved saccharification in RG stems, but a chemical shift to greater S:G ratios in RG stem lignin was also observed. Similarities in cellulose content and structure by XRD-analysis support the correlation between increased saccharification properties and reduced lignin instead of changes in the cellulose composition and/or structure.ConclusionAntithetic lignin accumulation was observed in the RG mutant leaf-and stem-tissue, which resulted in greater saccharification efficiency in the RG stem and differential thermochemical product yield in high lignin leaves. Thus, the red leaf coloration of the RG mutant represents a potential marker for improved conversion of stem cellulose to fermentable sugars in the C4 grass Sorghum.