Susceptibility to endoglycosidase F and H at the individual glycosylation sites of mouse thyrotropin and free alpha-subunits.
Susceptibility to endoglycosidase F and H at the individual glycosylation sites of mouse thyrotropin and free alpha-subunits.
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小鼠促甲状腺素和游离 α 亚基的各个糖基化位点对糖苷内切酶 F 和 H 的敏感性。
DOI：
10.1055/s-2007-1004924
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Magner,JA
中科院分区：
文献类型：
--
作者：
Miura,Y;Perkel,VS;Magner,JA
我们研究了小鼠 TSH 和游离 α 亚基各个糖基化位点对寡糖内切糖苷酶 F 和 H 的不同敏感性。将小鼠促甲状腺肿瘤组织与 D-[2-3 H] 甘露糖一起孵育 6 小时。 [3 H] Man 标记的 TSH 和游离 α 亚基使用特定抗血清从匀浆中获得，并用内切糖苷酶 F 和 H 在天然状态下或在热变性和去垢剂存在下还原后进行消化。然后通过反相 HPLC 分析消化产物的胰蛋白酶片段，从而可以确定内切糖苷酶在各个糖基化位点的作用。
We have studied the differential susceptibility to endoglycosidase F and H of oligosaccharides at the individual glycosylation sites of mouse TSH and free α-subunits. Mouse thyrotropic tumor tissue was incubated with D-[2-3 H] mannose for 6h.[3 H] Man-labeled TSH and free α-subunits were obtained from homogenates using specific antisera and were digested with endoglycosidase F and H in their native states or after heat-denaturation and reduction in the presence of detergents. Tryptic fragments of the digestion products were then analyzed by reverse phase HPLC so that effects of endoglycosidase at the individual glycosylation sites could be determined.