Rapid analysis of aerosol drugs using nano extractive electrospray ionization tandem mass spectrometry
Rapid analysis of aerosol drugs using nano extractive electrospray ionization tandem mass spectrometry
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DOI：
10.1039/b923991j
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发表时间：
2010-01-01
期刊：
影响因子：
4.2
通讯作者：
Chen, Huanwen
中科院分区：
文献类型：
--
作者：
Gu, Haiwei;Hu, Bin;Chen, Huanwen
气雾剂药物在市场上的药物制剂中占很大份额。建立了一种新的纳米萃取电喷雾电离质谱（nanoEESI-MS）快速分析气溶胶药物样品的方法。在没有任何样品预处理的情况下，将气溶胶药物手动喷射到由纳米电喷雾发射器产生的初级离子羽流中，以在环境条件下直接电离。将感兴趣的分析物离子引导到离子阱质谱仪中进行串联质谱分析。采用nanoEESI-MS对硝酸益康唑、丙酸倍氯米松、水杨酸甲酯和苯海拉明的二元混合物、特布他林和沙丁胺醇等气雾剂中的有效成分进行了快速检测，单次样品分析时间在1.2 s内完成。串联质谱法用于确认每个气溶胶药物样品中重要化合物的鉴定。合理的相对标准偏差（RSD = 6.39%，n = 13）和可接受的灵敏度（10 ppt，100 μ L）的沙丁胺醇气雾剂样品，这表明nanoEESI-MS分析复杂的药物样品的定量能力。将该方法应用于沙丁胺醇热分解过程的研究，可以方便地跟踪沙丁胺醇的降解动力学。我们的数据表明，nanoEESI串联质谱是一种快速，灵敏的技术，用于分析气雾剂药物制剂，显示出良好的应用前景，在药理学研究和现场分析气雾剂药物的市场。
Aerosol drugs dominate a significant share of pharmaceutical preparations on the market. A novel sensitive method utilizing nano extractive electrospray ionization mass spectrometry (nanoEESI-MS) has been developed for the rapid analysis of aerosol drug samples with quantitative information. Without any sample pretreatment, aerosol drugs were manually sprayed into the primary ion plume created by a nano electrospray emitter for direct ionization under ambient conditions. The analyte ions of interest were guided into an ion trap mass spectrometer for tandem mass analysis. The active ingredients of various aerosol drugs, such as econazole nitrate, beclomethasone dipropionate, binary mixture of methyl salicylate and diphenhydramine, terbutaline, and salbutamol, were rapidly detected using nanoEESI-MS. A single sample analysis could be completed within 1.2 s. Tandem mass spectrometry was used to confirm the identification of important compounds in each aerosol drug sample. Reasonable relative standard deviation (RSD = 6.39%, n = 13) and acceptable sensitivity (10 ppt, 100 mu L) were found for the salbutamol aerosol sample, which suggests that nanoEESI-MS has the quantitative capacity for analyzing complex pharmaceutical samples. This method was further extended to study the thermal decomposition process of salbutamol, showing that the degradation kinetics of salbutamol can be conveniently tracked. Our data demonstrate that nanoEESI tandem mass spectrometry is a fast and sensitive technique for the analysis of aerosol drug preparations, showing promising applications in pharmacology studies and in situ analysis of aerosol drugs on the market.