Self-assembly of polydimethylsiloxane structures from 2D to 3D for bio-hybrid actuation
Self-assembly of polydimethylsiloxane structures from 2D to 3D for bio-hybrid actuation
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DOI：
10.1088/1748-3190/10/5/056001
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发表时间：
2015-10-01
影响因子：
3.4
通讯作者：
Menciassi, A.
中科院分区：
文献类型：
--
作者：
Vannozzi, L.;Ricotti, L.;Menciassi, A.
这项工作的目的是证明一种新的方法的可行性，用于开发的3D自组装聚二甲基硅氧烷结构，被用作工程柔性矩阵的生物混合驱动。我们描述了工程双层的制造，组织在一个3D架构的应力诱导的滚动膜技术的手段。这样的结构被提供有特定的表面形貌，用于膜卷起后的细胞排列和细胞存活。我们报告了先进的有限元模型模拟的结果，预测系统的行为方面的整体收缩，诱导的肌肉细胞接种在膜上的收缩活动。然后，我们在体外测试与原代心肌细胞的结构，以评估真实的生物致动器收缩，从而验证模拟结果。在后期阶段，我们通过共价结合聚合物表面上的蛋白质，为样品提供了稳定的纤连蛋白涂层，从而使C2C12骨骼肌细胞（一种更可控的细胞类型）能够长期培养。这些测试揭示了在卷起的结构上的细胞活力和排列，以及细胞分化和在聚合物表面上形成多核和定向肌管的能力，也由成纤维细胞饲养层支撑。我们的研究结果突出了开发三维滚动PDMS结构的可能性，其特征在于不同的机械性能，作为新型生物混合致动器。
This work aims to demonstrate the feasibility of a novel approach for the development of 3D self-assembled polydimethylsiloxane structures, to be used as engineered flexible matrices for bio-hybrid actuation. We described the fabrication of engineered bilayers, organized in a 3D architecture by means of a stress-induced rolling membrane technique. Such structures were provided with ad hoc surface topographies, for both cell alignment and cell survival after membrane rolling. We reported the results of advanced finite element model simulations, predicting the system behavior in terms of overall contraction, induced by the contractile activity of muscle cells seeded on the membrane. Then, we tested in vitro the structure with primary cardiomyocytes to evaluate the real bio-actuator contraction, thus validating the simulation results. At a later stage, we provided the samples with a stable fibronectin coating, by covalently binding the protein on the polymer surface, thus enabling long-term cultures with C2C12 skeletal muscle cells, a more controllable cell type. These tests revealed cell viability and alignment on the rolled structures, but also the ability of cells to differentiate and to form multinucleated and oriented myotubes on the polymer surface, also supported by a fibroblast feeder layer. Our results highlighted the possibility of developing 3D rolled PDMS structures, characterized by different mechanical properties, as novel bio-hybrid actuators.