Effect of sequence depth and length in long-read assembly of the maize inbred NC358
Effect of sequence depth and length in long-read assembly of the maize inbred NC358
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DOI：
10.1038/s41467-020-16037-7
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发表时间：
2020-05-08
影响因子：
16.6
通讯作者：
Ware, Doreen
中科院分区：
文献类型：
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作者：
Ou, Shujun;Liu, Jianing;Ware, Doreen
长读长数据和支架构建技术的改进使得复杂基因组的参考质量组装能够快速生成。然而，对关键序列深度和读长的评估对于分配有限资源是很重要的。为此，我们使用PacBio数据集为玉米自交系NC358的复杂基因组生成了8个组装结果，这些数据集的基因组深度范围从20到75倍，N50子读长为11 - 21kb。具有……的组装结果
Improvements in long-read data and scaffolding technologies have enabled rapid generation of reference-quality assemblies for complex genomes. Still, an assessment of critical sequence depth and read length is important for allocating limited resources. To this end, we have generated eight assemblies for the complex genome of the maize inbred line NC358 using PacBio datasets ranging from 20 to 75xgenomic depth and with N50 subread lengths of 11-21kb. Assemblies with