Rapid serodiagnosis of Mycobacterium avium-intracellulare complex infection by ELISA with cord factor (Trehalose 6, 6′-dimycolate), and serotyping using the glycopeptidolipid antigen
Rapid serodiagnosis of Mycobacterium avium-intracellulare complex infection by ELISA with cord factor (Trehalose 6, 6′-dimycolate), and serotyping using the glycopeptidolipid antigen
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DOI：
10.1111/j.1348-0421.1998.tb02341.x
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发表时间：
1998-01-01
影响因子：
2.6
通讯作者：
Yano, I
中科院分区：
文献类型：
--
作者：
Enomoto, K;Oka, S;Yano, I
鸟胞内分枝杆菌复合体（Mycobacteriumavium-intracellulare complex，MAC）是获得性免疫缺陷综合征（acquired immunodeficiency syndrome，AIDS）患者最重要的机会致病菌之一。本研究旨在探讨以海藻糖6，6 '-二霉菌酸酯（trehalose 6，6'-dimycolate，TDM）为抗原的酶联免疫吸附试验（enzyme-linked immunosorbent assay，ELISA）能否用于MAC感染的快速血清学诊断。我们还确定了MAC的血清型ELISA使用特定的糖肽脂（GPL）抗原。为了证实我们的发现，还测试了从MAC感染患者分离的菌株的型特异性GPL的薄层色谱（TLC）行为。对40例MAC感染患者和30例健康对照进行了检测。40例MAC感染者中有32例血清抗MAC TDM抗体滴度高于抗MTB TDM抗体滴度，而30例健康对照血清对MAC TDM和MTB TDM均无反应。GPLELISA结果显示，40例MAC感染者中有20例血清抗4型GPL，3例抗8型GPL，1例抗16型GPL。对40株MAC分离株进行薄层层析（TLC）分析，结果表明：血清4型16株，血清8型5株，血清16型2株。对大多数患者，GPL-ELISA结果与TLC分析结果雅阁。并且使用MAC型特异性GPL的补充ELISA测试给出了关于MAC血清型的额外详细信息。
Mycobacterium avium-intracellulare complex (MAC) is one of the most important opportunistic pathogens, particularly in patients with acquired immunodeficiency syndrome (AIDS), The aim of this study was to determine whether an enzyme-linked immunosorbent assay (ELISA) using trehalose 6, 6'-dimycolate (TDM) as an antigen can be used for the rapid serodiagnosis of MAC infection. We also identified MAC serotypes by ELISA using serotype-specific glycopeptidolipid (GPL) antigen. To confirm our findings, the thin-layer chromatographic (TLC) behavior of serotype-specific GPL of the strains isolated from MAC-infected patients was also tested. Forty patients infected with MAC and 30 healthy controls were tested. Thirty-two of the 40 MAC-infected patients had higher titers of serum antibodies against MAC TDM than against MTB TDM, while all 30 healthy control sera were unreactive to MAC TDM and MTB TDM, Results of the GPL ELISA indicated that 20 of the 40 MAC-infected patients' sera were reactive against serotype 4 GPL, 3 against serotype 8 GPL, and 1 against serotype 16 GPL, A TLC analysis of the GPL of the 40 MAC isolates showed that 16 strains mere of serotype 4, 5 of serotype 8, and 2 of serotype 16, Results of the GPL ELISA were in good accord with those of the TLC analysis for most patients, Our findings suggest that ELISA using TDM is useful for rapid serodiagnosis of MAC infection, and that complementary ELISA testing using serotype-specific GPL gives additional detailed information concerning MAC serotypes.