The juxtamembrane domains of Arabidopsis CERK1, BAK1, and FLS2 play a conserved role in chitin-induced signaling
The juxtamembrane domains of Arabidopsis CERK1, BAK1, and FLS2 play a conserved role in chitin-induced signaling
复制标题
拟南芥 CERK1、BAK1 和 FLS2 的近膜结构域在几丁质诱导的信号传导中发挥保守作用
DOI：
10.1111/jipb.12847
复制
发表时间：
--
影响因子：
11.4
通讯作者：
Liu Bing
中科院分区：
文献类型：
--
作者：
Zhou Qi;Liu Jun;Wang Jingyi;Chen Sufen;Chen Lijuan;Wang Jinfa;Wang Hong Bin;Liu Bing
拟南芥CERK1是几丁质信号转导途径中一种重要的受体样蛋白。CERK1的膜旁(JM)结构域调节该受体的激酶活性。在这里，我们证明了LysM-RLKs、CERK1和OsCERK1的JM结构域在拟南芥几丁质信号的激活中起着功能保守的作用。CERK1和OsCERK1的JM结构域的C末端对于它们的功能是必不可少的。此外，在用非同源的RLK、BAK1(CJBA)或Fls2(CJF1)替换CERK1的JM结构域后，嵌合的CERK1受体在拟南芥中保持了激活几丁质信号的能力。有趣的是，CJBA和CJF1的异源表达并没有诱导烟草的细胞死亡。这些结果表明，CERK1、BAK1和Fls2的JM结构域通过与序列同源性无关的机制在甲壳素信号转导中起着保守的作用。
Arabidopsis thalianaCERK1 is an essential receptor‐like kinase in the chitin signal transduction pathway. The juxtamembrane (JM) domain of CERK1 regulates the kinase activity of this receptor. Here we demonstrate that the JM domains of LysM‐RLKs, CERK1, and OsCERK1 play a functionally conserved role in the activation of chitin signaling inArabidopsis. The C‐termini of the JM domains of both CERK1 and OsCERK1 are indispensable for their function. Moreover, after replacing the JM domain of CERK1 with that of the nonhomologous RLK, BAK1 (CJBa) or FLS2 (CJFl), the chimeric CERK1 receptors maintained their ability to activate chitin signaling inArabidopsis. Interestingly, the heterologous expression of CJBa and CJFl did not induce cell death inNicotiana benthamianaleaves. These results suggest that the JM domains of CERK1, BAK1, and FLS2 play a conserved role in chitin signaling via a mechanism not related to sequence homology.