REDUCTION IN HEAT SHOCK PROTEIN 90 CORRELATES TO NEURONAL VULNERABILITY IN THE RAT PIRIFORM CORTEX FOLLOWING STATUS EPILEPTICUS
REDUCTION IN HEAT SHOCK PROTEIN 90 CORRELATES TO NEURONAL VULNERABILITY IN THE RAT PIRIFORM CORTEX FOLLOWING STATUS EPILEPTICUS
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DOI：
10.1016/j.neuroscience.2013.09.050
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发表时间：
2013-12-26
期刊：
影响因子：
3.3
通讯作者：
Kang, T. -C.
中科院分区：
文献类型：
--
作者：
Kim, Y. -J.;Kim, J. -Y.;Kang, T. -C.
在本研究中，我们解决了热休克蛋白（HSP）70和HSP90在大脑区域表达的不同模式是否代表对癫痫持续状态（SE）的区域特异性反应的问题，以更好地了解热休克蛋白在致癫痫损伤中的作用。 SE 后 12 小时至 1 周，CA3 锥体细胞和齿状颗粒细胞中的 HSP70 免疫反应性增加。 SE 后梨状皮层 (PC) 内神经元的 HSP70 免疫反应性短暂增加。线性回归分析显示NeuN 强度和HSP70 强度之间没有相关性。与 HSP70 相比，SE 后 4 天至 4 周，CA1-3 锥体细胞中的 HSP90 免疫反应性降低。此外，SE 后 12 小时至 4 周，PC 神经元中的 HSP90 免疫反应性降低。线性回归分析显示NeuN的强度与HSP90的强度之间呈正比例关系。因此，这些发现表明 HSP90 降解可能与神经元对 SE 损伤的脆弱性密切相关。 (C) 2013 国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
In the present study, we addressed the question of whether the distinct patterns of heat shock protein (HSP) 70 and HSP90 expressions in the brain region represents the regional specific responses to status epilepsticus (SE) in an effort to better understand the role of HSPs in epileptogenic insult. HSP70 immunoreactivity was increased in CA3 pyramidal cells as well as dentate granule cells at 12 h-1 week after SE. HSP70 immunoreactivity was transiently increased in neurons within the piriform cortex (PC) following SE. Linear regression analysis showed no correlation between the intensity of NeuN and that of HSP70. In contrast to HSP70, HSP90 immunoreactivity was decreased in CA1-3 pyramidal cells at 4 days-4 weeks after SE. In addition, HSP90 immunoreactivity was decreased in PC neurons at 12 h-4 weeks after SE. linear regression analysis showed a direct proportional relationship between the intensity of NeuN and that of HSP90. Therefore, these findings suggest that HSP90 degradation may be closely related to neuronal vulnerability toSEinsult. (C) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.