Modular microfluidic reactor and inline filtration system for the biocatalytic synthesis of chiral metabolites
Modular microfluidic reactor and inline filtration system for the biocatalytic synthesis of chiral metabolites
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DOI：
10.1016/j.molcatb.2011.12.010
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发表时间：
2012-05-01
影响因子：
--
通讯作者：
Szita, Nicolas
中科院分区：
文献类型：
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作者：
O'Sullivan, Brian;Al-Bahrani, Homam;Szita, Nicolas
生物催化合成由于其高反应特异性和选择性等优点，已成为工业合成高价值化合物的一种非常有用的方法。然而，设计一个生物催化过程可能是复杂和耗时的。本文介绍了一种模块化微流控反应器和在线过滤系统，用于快速和小规模的生物催化反应评价。该系统将底物与生物催化剂结合在自由溶液中，培养两种成分，直到完全转化为产品，然后提取产品。该系统已应用于转酮醇酶催化羟丙酮酸（HPA）和乙醇醛（GA）生成l -赤藓糖的反应，表明底物完全转化为产物，酶完全保留，总产率约为65%。突变体转酮醇酶将HPA和丙醛完全转化为(3S)-1,3-二羟基戊烷-2 -1，进一步证明了微流控系统在有机合成中的适用性。(C) 2012 Elsevier B.V.版权所有
Biocatalytic synthesis is now well established amongst catalytic methodologies as an extremely useful approach for the industrial synthesis of high-value compounds, due to its many advantages such as high reaction specificity and selectivity. However, engineering a biocatalytic process can be complex and time-consuming. This paper presents a modular microfluidic reactor and in-line filtration system for the rapid and small-scale evaluation of biocatalytic reactions. The system combines a substrate with a biocatalyst in free solution, incubates the two components until full conversion to product has been achieved, before extracting the product. The system has been applied to the transketolase-catalysed reaction of hydroxypyruvate (HPA) and glycolaldehyde (GA) to L-erythrulose, demonstrating complete conversion of substrate to product, complete retention of the enzyme and an overall yield of approximately 65%. The complete conversion of HPA and propanal to (3S)-1,3-dihydroxypentan-2-one with a mutant transketolase further demonstrated the applicability of the microfluidic system for organic synthesis. (C) 2012 Elsevier B.V. All rights reserved.