THE COLLECTION AND ANALYSIS OF FLUID FROM SINGLE NEPHRONS OF THE MAMMALIAN KIDNEY
THE COLLECTION AND ANALYSIS OF FLUID FROM SINGLE NEPHRONS OF THE MAMMALIAN KIDNEY
复制标题
哺乳动物肾脏单个肾单位液体的收集和分析
DOI：
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发表时间：
1941
期刊：
影响因子：
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通讯作者：
M. Macdowell
中科院分区：
文献类型：
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作者：
A. M. Walker;P. A. Bott;J. Oliver;M. Macdowell
已经开发出从哺乳动物肾脏的单个肾小球和肾小管中收集液体的方法（1）。因此可以收集的流体量虽然很小（从0.03到0.70 c. mm），足以通过超微方法进行定量分析（2），并且已经在大鼠、豚鼠和负鼠的92个系列实验中进行了此类分析。实验的目的是了解液体沿着小管从肾小球流向输尿管时发生的一些变化，并确定这些变化的部位，同样类型的实验在两栖动物的肾脏中获得了相当多的信息（3）。在本例中，它证实了现有的肾小球功能的想法，建立了葡萄糖重吸收的网站，并阐明了近端小管中发生的液体重吸收的程度和类型。Anal@的方法。除钠的分析方法外，其他分析方法都是结合两栖动物的实验设计的，并在出版物中有充分的描述。它们在小玻璃毛细管中进行。以下段落总结了与本研究相关的原始技术和细节的变化。
Methods have been developed for the collection of fluid from single glomeruli and tubules of the mammalian kidney (1). The amounts of fluid which can thus be collected, though small (from 0.03 to 0.70 c.mm.), were sufficient for quantitative analysis by ultramicro methods (2), and such analyses have been performed in a series of 92 experiments upon rats, guinea pigs and opossums. It was the design of the experiments to learn some of the changes which occur as fluid descends a tubule from the glomerulus toward the ureter and to establish the site of these changes, the same type of experimentation which has yielded considerable information in the amphibian kidney (3). In the present instance it has confirmed existing ideas of glomerular function, established the site of glucose reabsorption, and shed some light on the extent and type of fluid reabsorption which occurs in the proximal tubule. Methods of anal@. The analytical methods, excepting that for sodium, were devised in connection with the experiments upon amphibia and are fully described in the publications to which reference will be made. They were carried out in small glass capillary tubes. Changes in the original techniques and details pertinent to the present investigation are summarized in the following paragraphs.