Constructing a Synthetic Metabolic Pathway in Escherichia Coli to Produce the Enantiomerically Pure (R, R)-2,3-Butanediol
Constructing a Synthetic Metabolic Pathway in Escherichia Coli to Produce the Enantiomerically Pure (R, R)-2,3-Butanediol
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在大肠杆菌中构建合成代谢途径以生产对映体纯的 (R, R)-2,3-丁二醇。
DOI：
10.1002/bit.25512
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发表时间：
2015-05-01
影响因子：
3.8
通讯作者：
Huang, He
中科院分区：
文献类型：
--
作者：
Ji, Xiao-Jun;Liu, Lu-Gang;Huang, He
对映体纯的（R，R）-2，3-丁二醇由于其特殊的手性基团和空间构型而具有独特的应用。目前，其化工生产路线存在诸多局限性。此外，没有天然微生物可以积累对映体纯度超过99%的（R，R）-2，3-丁二醇。在此，我们构建了一个在大肠杆菌中合成对映体纯的（R，R）-2，3-丁二醇的合成代谢途径。发酵结果表明，合成代谢途径的引入使碳流重新分配到中性的（R，R）-2，3-丁二醇，从而保护了菌株免受乙酸的抑制。此外，传统的异丙基β-D-硫代半乳糖苷（IPTG）诱导对重组E.大肠杆菌，这可能是由于蛋白质负担。在不添加IPTG的情况下，通过流加培养，（R，R）-2，3-丁二醇的产量达到115 g/L。大肠杆菌中获得了对映体纯度大于99%的目标产物，适合于中试生产。Biotechnol. Bioeng. 2015;112：1056-1059. (c)2014 Wiley Periodicals，Inc.
Enantiomerically pure (R, R)-2,3-butanediol has unique applications due to its special chiral group and spatial configuration. Currently, its chemical production route has many limitations. In addition, no native microorganisms can accumulate (R, R)-2,3-butanediol with an enantio-purity over 99%. Herein, we constructed a synthetic metabolic pathway for enantiomerically pure (R, R)-2,3-butanediol biosynthesis in Escherichia coli. The fermentation results suggested that introduction of the synthetic metabolic pathway redistributed the carbon fluxes to the neutral (R, R)-2,3-butanediol, and thus protected the strain against the acetic acid inhibition. Additionally, it showed that the traditionally used isopropyl beta-D-thiogalactoside (IPTG) induction displayed negative effect on (R, R)-2,3-butanediol biosynthesis in the recombinant E. coli, which was probably due to the protein burden. With no IPTG addition, the (R, R)-2,3-butanediol concentration reached 115g/L by fed-batch culturing of the recombinant E. coli, with an enantio-purity over 99%, which is suitable for the pilot-scale production. Biotechnol. Bioeng. 2015;112: 1056-1059. (c) 2014 Wiley Periodicals, Inc.