Total and high-molecular weight adiponectin in relation to metabolic variables at baseline and in response to an exercise treatment program -: Comparative evaluation of three assays
Total and high-molecular weight adiponectin in relation to metabolic variables at baseline and in response to an exercise treatment program -: Comparative evaluation of three assays
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DOI：
10.2337/dc06-1362
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发表时间：
2007-02-01
期刊：
影响因子：
16.2
通讯作者：
Mantzoros, Christos S.
中科院分区：
文献类型：
--
作者：
Blueher, Matthias;Fasshauer, Mathias;Mantzoros, Christos S.
目的-脂联素是一种脂肪细胞分泌的激素，以多种多聚体形式在血清中循环。与总脂联素相比，高分子量（HMW）脂联素被认为可以更好地预测人体代谢参数和胰岛素敏感性。我们的目的是比较总脂联素和 HMW 脂联素作为基线和运动干预后代谢变量和胰岛素敏感性的预测因子。 研究设计和方法 - 我们从基线和 4 周训练后具有正常糖耐量 (n = 20)、糖耐量受损 (IGT) (n = 20) 或 2 型糖尿病 (n = 20) 的 60 名男性和女性中获取血液样本，以测量代谢变量。使用市售的检测方法，我们使用 LINCO、Mediagnost 和 ALPCO 检测方法测量血浆总脂联素，并使用 ALPCO 检测方法测量 HMW 脂联素。 结果：HMW 脂联素和总脂联素 (ALPCO) 具有相似的预测胰岛素抵抗存在的能力。通过根免疫测定 (LINCO) 和酶联免疫吸附测定 (ELISA) (Mediagnost) 测量的总脂联素与基线时的胰岛素敏感性 (P < 0.01) 和血脂谱 (P < 0.01) 相关性最强，显示脂联素水平有更大的改善 (P < 0.001)，与运动训练中血脂测量的改善关系更密切 (P < 0.01)，并且与使用 ALPCO ELISA 测量的总脂联素或 HMW 相比，可以更准确地预测胰岛素抵抗和 IGT。结论 - 这些结果并不支持 HMW 在评估基线代谢变量或对体育训练的反应方面优于总脂联素（使用当前可用的测定法测量）。此外，商业上可用的总脂联素检测方法预测代谢变量的能力存在显着差异。
OBJECTIVE-Adiponectin, an adipocyte-secreted hormone, circulates in the serum in several multimeric forms. Compared with total adiponectin, high-molecular weight (HMW) adiponectin has been suggested to be a better predictor of metabolic parameters and insulin sensitivity in humans. Our objective was to compare total adiponectin with HMW adiponectin as predictors of metabolic variables and insulin sensitivity at both baseline and after an exercise intervention.RESEARCH DESIGN AND METHODS-We obtained blood samples from 60 men and women with normal glucose tolerance (n = 20), impaired glucose tolerance (IGT) (n = 20), or type 2 diabetes (n = 20) at baseline and after 4 weeks Of training to measure metabolic variables. Using commercially available 'assays, we measured plasma total adiponectin using LINCO, Mediagnost, and ALPCO assays and HMW adiponectin using an ALPCO assay.RESULTS-HMW adiponectin and total adiponectin (ALPCO) had similar ability to predict the presence of insulin resistance. Total adiponectin, as measured by radicimmunoassay (LINCO) and enzyme-linked immunosorbent assay.(ELISA) (Mediagnost), correlated most strongly with measures of insulin sensitivity (P < 0.01) and lipid profile (P < 0.01.) at baseline, showed greater improvements of adiponectin levels (P < 0.001), was more closely associated with improvements of lipid measures with exercise training (P < 0.01), and more accurately predicted insulin resistance and IGT in comparison with total adiponectin or HMW measured with the ALPCO ELISA.CONCLUSIONS-These results do not support the superiority of HMW over total adiponectin (measured using currently available assays) in assessing metabolic variables at baseline or in response to physical training. Moreover, there are significant differences in the ability of commercially available assays for total adiponectin to predict metabolic variables.