New ultraviolet (340 nm) method for assay of uric acid in serum or plasma.
New ultraviolet (340 nm) method for assay of uric acid in serum or plasma.
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用于测定血清或血浆中尿酸的新紫外（340 nm）方法。
DOI：
10.1093/clinchem/24.4.562
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发表时间：
1978
影响因子：
9.3
通讯作者：
L. Stong
中科院分区：
文献类型：
--
作者：
R. C. Trivedi;L. Rebar;K. Desai;L. Stong
我们提出了一种在340 nm波长处测定尿酸的新的酶促方法，克服了目前常用的比色法和酶法的一些缺点。在这里，尿酸被催化氧化成尿囊素和过氧化氢。过氧化氢在过氧化氢酶的催化下与乙醇反应生成乙醛和水，乙醛在乙醇脱氢酶的催化下被NADH还原为乙醇。NADH氧化引起的340 nm处吸光度的下降与样品中尿酸的浓度成正比。测量吸光度在20到200之间的变化，S消除了血清空白测量的需要。吸光度和浓度与每升120毫克尿酸呈线性关系。并与直接测定尿酸在293 nm波长处分解的尿酸酶法进行了比较。47例患者血清的测定结果可用线性方程Y340=1.0078×293+0.122(r=0.9984)表示。
We propose a novel enzymatic method for assay of uric acid at 340 nm, which eliminates several disadvantages of both the colorimetric and enzymatic methods now in common use. Here, uric acid is catalytically oxidized to allantoin and hydrogen peroxide. The peroxide is reacted with ethanol in the presence of catalase to form acetaldehyde and water, and the acetaldehyde is reduced by NADH in the presence of alcohol dehydrogenase to ethanol. The decrease in absorbance at 340 nm caused by oxidation of NADH is directly proportional to the concentration of uric acid in the sample. Measurement of the change in absorbance between 20 and 200 s eliminates the need for a serum blank measurement. Absorbance and concentration are linearly related to 120 mg of uric acid per liter. The new method was compared with the uricase method in which decomposition of uric acid at 293 nm is directly measured. The results for the 47 patients' sera so examined can be expressed by the linear equation y340 = 1.0078x293 + 0.122 (r = 0.9984).