Biological control of the European cockchafer: persistence of Beauveria brongniartii after long-term applications in the Euroregion Tyrol
Biological control of the European cockchafer: persistence of Beauveria brongniartii after long-term applications in the Euroregion Tyrol
复制标题
欧洲金龟子的生物防治：在欧洲蒂罗尔州长期施用后布氏白僵菌的持久性
DOI：
10.1007/s10526-015-9671-6
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发表时间：
2015
期刊：
影响因子：
2.5
通讯作者：
H. Strasser
中科院分区：
文献类型：
--
作者：
J. Mayerhofer;J. Enkerli;R. Zelger;H. Strasser
基于昆虫病原真菌布氏白僵菌（Beauveria brongniartii（Sacc.）Petch（子囊菌门：在东蒂罗尔、北蒂罗尔和南蒂罗尔（奥地利和意大利），在24年的时间内以不同的比率和时间点施用Hypocreales）菌株BIP12，以控制欧洲金龟子（MelolonthamelolonthaL.）。和M. castaniF.）。博韦里亚普密度进行了评估和Beauveriaspp.分离物的特征在于在种和基因型水平上使用Bloc基因间区序列和简单重复序列标记分析。在41%的治疗部位检测到BIPpyramid2，主要是在采样前四年内至少使用过一次真菌的部位。在最后一次治疗后15年，在一个部位检测到BIPs2。结果表明，应重复使用，以实现良好的持久性的生物控制剂，这表明，尽管密集的应用，不同的种群的B。勃龙尼亚群落可以在这些栖息地共存。
Melocont®Pilzgerste, a commercial product based on the entomopathogenic fungusBeauveria brongniartii(Sacc.) Petch (Ascomycota: Hypocreales) strain BIPESCO 2, was applied at different rates and time points over a period of 24 years in East-, North- and South Tyrol (Austria and Italy) to control the European cockchafer (MelolonthamelolonthaL. andM. hippocastaniF.).Beauveriaspp. density was assessed andBeauveriaspp. isolates were characterized at species and genotype level using Bloc intergenic region sequence and simple sequence repeat marker analyses. BIPESCO 2 was detected at 41 % of the treated sites, predominantly at sites where the fungus was applied at least once during the four years prior to sampling. At one site BIPESCO 2 was detected 15 years after the last treatment. Results showed that applications should be repeated to achieve good persistence of the biological control agent, and it indicated that despite intensive applications diverse populations ofB. brongniartiiorBeauveriaspp. consortia can coexist in these habitats.