Electrophoretic karyotype of intracellular yeast-like symbiotes in rice planthoppers and anobiid beetles.
Electrophoretic karyotype of intracellular yeast-like symbiotes in rice planthoppers and anobiid beetles.
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DOI：
10.1006/jipa.1995.1018
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发表时间：
1995-03
影响因子：
3.4
通讯作者：
H. Noda;N. Kawahara
中科院分区：
文献类型：
--
作者：
H. Noda;N. Kawahara
用脉冲场凝胶电泳法分离了三种稻飞虱和两种拟步甲胞内酵母样共生体（YLS）的染色体DNA分子。褐飞虱、白背飞虱和灰飞虱的可能染色体数目分别为4、4和5，基于单个染色体大小的DNA相对于粟酒裂殖酵母、汉逊酵母和酿酒酵母染色体大小标准的迁移，暂定基因组大小分别为17.3、17.6和20.1 Mbp。卷须石松和圆锥坚甲的染色体数目分别为11和15，总基因组大小分别为20.9和15.1Mbp。Southern杂交鉴定携带核糖体RNA基因的染色体。YLS的染色体组构和基因组大小与非共生酵母和真菌相似。
Chromosomal DNA molecules of intracellular yeast-like symbiotes (YLS) of three species of rice planthoppers and two species of anobiid beetles have been separated by pulsed-field gel electrophoresis. Probable chromosome numbers of Nilaparvata lugens, Sogatella furcifera, and Laodelphax striatellus, were 4, 4, and 5, respectively, and tentative genome sizes were 17.3, 17.6, and 20.1 Mbp, respectively, based upon migration of individual chromosome-sized DNA relative to the size standards of Schizosaccharomyces pombe, Hansenula wingei, and Saccharomyces cerevisiae chromosomes. Chromosome numbers of Lasioderma serricorne and Stegobium paniceum were 11 and 15, respectively, and total genome sizes were 20.9 and 15.1 Mbp, respectively. The chromosomes carrying ribosomal RNA genes were identified by Southern blot analysis. Chromosomal organization and the genome size of YLS were similar to those of nonsymbiotic yeasts and fungi.