Effect of acetylcholine on Cl- and Na+ fluxes across dog tracheal epithelium in vitro.
Effect of acetylcholine on Cl- and Na+ fluxes across dog tracheal epithelium in vitro.
复制标题
乙酰胆碱对体外狗气管上皮的 Cl- 和 Na 通量的影响。
DOI：
10.1152/ajplegacy.1976.231.5.1546
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
J. Nadel
中科院分区：
文献类型：
--
作者：
M. Marin;B. Davis;J. Nadel
通过Cl-向管腔主动转运和Na+远离管腔主动转运，在犬气管上皮上产生电势差。本研究探讨了乙酰胆碱对短路电流，电位差，电阻和36 Cl和24 Na跨片犬气管上皮细胞安装在Ussing型腔室测量通量的影响。在短路条件下，乙酰胆碱（5 × 10（-5）M）显著增加了Cl-（n = 7）从+1.7 +/- SE 0.5到+3.3 +/- SE 0.5 mueq/cm 2- h，Na+（n = 7）从-0.8 +/- SE 0.2到+0.5 +/- SE 0.2 mueq/cm 2- h流向管腔的净离子通量。在开路条件下，乙酰胆碱（5 × 10（-5）M）显著增加了Cl-（n = 6）向管腔的单向通量，从4.7 +/- SE 1.3增加到5.9 +/- SE 1.4 mueq/cm 2- h，而其他测量的通量没有显著变化，表明净Cl-通量向管腔增加。硫酸阿托品（10（-8）M）阻止对乙酰胆碱（5 × 10（-5）M）的反应。由于乙酰胆碱增加的离子通量可介导水分泌到气道腔中，并且这种分泌可对呼吸纤毛跳动所通过的液体的物理性质具有重要影响。
Electrical potential difference is generated across canine tracheal epithelium by active transport of Cl- toward and Na+ away from the lumen. The present study examines the effects of acetylcholine on short-circuit current, potential difference, resistance, and fluxes of 36Cl and 24Na measured across pieces of canine tracheal epithelium mounted in Ussing-type chambers. Under short-circuit conditions, acetylcholine (5 X 10(-5) M) increased significantly net ion flux toward the lumen of Cl- (n equals 7) from +1.7 +/- SE 0.5 TO +3.3 +/- SE 0.5 mueq/cm2 - h, and of Na+ (n equals 7) from -0.8 +/- SE 0.2 to +0.5 +/- SE 0.2 mueq/cm2 - h. Under open-circuit conditions, acetylcholine (5 X 10(-5) M) increased significantly the unidirectional flux of Cl- (n equals 6) toward the lumen from 4.7 +/- SE 1.3 to 5.9 +/- SE 1.4 mueq/cm2 - h, while the other measured fluxes did not change significantly, suggesting that net Cl- flux had increased toward the lumen. Atropine sulfate (10(-8) M) prevented the response to acetylcholine (5 X 10(-5) M). The increased ion flux due to acetylcholine may mediate water secretion into the airway lumen, and this secretion may have important effects on the physical properties of the liquid through which the respiratory cilia beat.