Chemoselective Synthesis of Folic Acid-Functionalized Magnetite Nanoparticles via Click Chemistry for Magnetic Hyperthermia
Chemoselective Synthesis of Folic Acid-Functionalized Magnetite Nanoparticles via Click Chemistry for Magnetic Hyperthermia
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DOI：
10.1021/cm803113e
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发表时间：
2009-04-14
影响因子：
8.6
通讯作者：
Yogo, Toshinobu
中科院分区：
文献类型：
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作者：
Hayashi, Koichiro;Moriya, Makoto;Yogo, Toshinobu
利用“点击化学”原理，以（III） 3-烯丙基乙酰丙酮铁（IAA）为原料，通过原位水解和配体修饰，合成了叶酸功能化的Fe3O4纳米颗粒（FA-Fe3O4 NPs）。FA的γ -羧酸成功地结合到Fe3O4 NPs的配体上，而没有失去叶酸（FA）的α -羧酸基团，叶酸（FA）与肿瘤细胞上表达的叶酸受体（FRs）有亲和力。Fe3O4 NPs是单分散的，其大小可以通过改变IAA水解条件来控制。直径为8 nm的FA-Fe3O4 NPs在室温下表现出超顺磁性和较高的磁化强度。阻断温度为220 K，磁化曲线在5 K时剩余率为19 emu/g，矫顽力为550 Oe。比吸收率（SAR）取决于FA-Fe3O4 NPs的尺寸和外加磁场的强度。在230 kHz交变磁场和100 Oe条件下，8 nm FA-Fe3O4 NPs的SAR为670 W/g。用FA对磁铁矿颗粒进行化学选择性表面修饰，产生了一种用于热疗治疗的新型癌症靶向系统。
Folic acid-functionalized Fe3O4 nanoparticles (FA-Fe3O4 NPs) were synthesized from iron(III) 3-allylacetylacetonate (IAA) through in situ hydrolysis and ligand modification, by applying the principle of "click chemistry". The gamma-carboxylic acid of FA was successfully bound to the ligand of the Fe3O4 NPs without the loss of the alpha-carboxylic acid group of folic acid (FA), which has an affinity for folate receptors (FRs) expressed on tumor cells. The Fe3O4 NPs were monodisperse, and their size was controlled by varying the conditions of IAA hydrolysis. The FA-Fe3O4 NPs, which had diameters of 8 nm, exhibited superparamagnetic behavior and a relatively high magnetization at room temperature. The blocking temperature was determined to be 220 K, and the magnetization curve exhibited a remanence of 19 emu/g and a coercivity of 550 Oe at 5 K. The specific absorption rate (SAR) was dependent on the size of the FA-Fe3O4 NPs and the strength of the applied magnetic field. The SAR of the 8-nm FA-Fe3O4 NPs was 670 W/g in a 230 kHz alternating magnetic field and 100 Oe. The chemoselective surface modification of magnetite particles with FA yielded a novel cancer-targeting system for use in hyperthermia treatment.