ALLOANTIBODY RESPONSE TO A STRONG TRANSPLANTATION ANTIGEN (AG-B) - QUANTITATIVE ASPECTS AND THYMUS DEPENDENCE OF RESPONSE
ALLOANTIBODY RESPONSE TO A STRONG TRANSPLANTATION ANTIGEN (AG-B) - QUANTITATIVE ASPECTS AND THYMUS DEPENDENCE OF RESPONSE
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DOI：
10.1097/00007890-197404000-00013
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发表时间：
1974-01-01
期刊：
影响因子：
6.2
通讯作者：
FORD, WL
中科院分区：
文献类型：
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作者：
ROLSTAD, B;WILLIAMS, AF;FORD, WL
目前尚不清楚对强效抗原(大鼠中由Ag-B决定的抗原)反应异常频繁的T淋巴细胞是否与同样高比例的反应B淋巴细胞平行。用血凝试验、淋巴细胞细胞毒试验和兔抗大鼠Fab标记抗体测定同种异体红细胞的总抗体，检测HO大鼠对DA抗原的同种异体抗体反应。结果发现：(1)静脉注射不同剂量的F1杂交瘤细胞，以3×106细胞诱导的抗体滴度最高.(2)同种异体抗体反应具有明显的胸腺依赖性.(3)(HO×DA)F1n皮肤移植物可使HO受体对第二次移植物的抗体应答明显高于对第一次移植物的抗体应答.(4)可获得的同种异体抗体的最高血清浓度低于对次佳剂量的绵羊红细胞产生的特异性抗体浓度.根据这一证据和其他证据，提出了一种情况，即B淋巴细胞对Ag-B抗原的反应频率可能并不例外；T淋巴细胞是唯一的“同种异体进行性”。
It is not known whether the exceptionally high frequency of T lymphocytes which respond to “strong” ulloantigens (Ag-B-determined antigens in the rat) is paralleled by an equally high proportion of responsive B lymphocytes. The alloantibody response of HO rats against DA antigens was measured by haemagglutination, lymphocyte cytotoxicity, and by use of a labeled rabbit anti-rat Fab to measure the total antibody bound to allogeneic erythrocytes. It was found that:(1) In response to graded doses of F 1 hybrid lymph node cells iv, the highest antibody titres were elicited by 3 X 10 6 cells.(2) The alloantibody response was markedly thymus-dependent.(3) An (HO X DA) F 1n skin graft primed an HO recipient to give a mucli higher antibody response to a second graft than to the first graft.(4) The highest serum concentration of alloantibody obtainable was less than the concentration of specific antibody produced in response to a suboptimal dose of sheep erythrocytes. On the basis of this and other evidence, a case is made that the frequency of B lymphocytes responsive to Ag-B antigens is probably not exceptional; T lymphocytes are uniquely “alloagressive.”