Synthesis and wound healing of alternating block polyurethanes based on poly(lactic acid) (PLA) and poly(ethylene glycol) (PEG)
Synthesis and wound healing of alternating block polyurethanes based on poly(lactic acid) (PLA) and poly(ethylene glycol) (PEG)
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DOI：
10.1002/jbm.b.33670
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发表时间：
2017-07-01
影响因子：
3.4
通讯作者：
Xu, Kaitian
中科院分区：
文献类型：
--
作者：
Li, Linjing;Liu, Xiangyu;Xu, Kaitian
以可生物降解的聚乳酸(PLA)和聚乙二醇(PEG)为原料，制备了交替嵌段聚氨酯(简称Pula-ALT-PEG)和无规嵌段聚氨酯(简称Pula-RAN-PEG)。结果表明，交替嵌段聚氨酯结构规整、可控，结晶度高，力学性能好，表面花纹多，表面粗糙。聚氨酯的吸水率在620-780%之间。以人胚胎肾(HEK293)细胞为实验材料，用CCK-8法评价了两亲嵌段聚氨酯(PU)(水静止角41.48~61.88)的细胞相容性。采用SD大鼠全层模型实验，以医用纱布为对照，对PU泡沫支架进行创面愈合评价。结果表明，PU组大鼠皮肤完全被新生上皮覆盖，无明显不良反应，PU敷料比医用纱布愈合更快、更好。组织学检查显示，PU敷料可抑制炎症细胞的渗透，促进成纤维细胞的增殖。研究还表明，Pula-ALT-PEG的愈合效果明显好于Pula-RAN-PEG。本研究表明，在不作进一步修饰的情况下，普通交替嵌段聚氨酯支架可以有效地促进伤口的愈合。(C)2016威利期刊公司。
Alternating block polyurethanes (abbreviated as PULA-alt-PEG) and random block polyurethanes (abbreviated as PULA-ran-PEG) based on biodegradable poly(lactic acid) (PLA) and poly(ethylene glycol) (PEG) were prepared. Results showed that alternating block polyurethane gives higher crystal degree, higher mechanical properties, more patterned and rougher surface than the random counterpart, due to the regular and controlled structure. Water absorptions of the polyurethanes were in the range of 620 to 780%. Cytocompatibility of the amphiphilic block polyurethanes (PU) (water static angle 41.48-61.88) was assessed by CCK-8 assay using human embryonic kidney (HEK293) cells. Wound healing evaluation of the PU foam scaffolds was carried out by full-thickness SD rat model experiment, with medical gauze as control. It was found that the skin of rat in PU groups was fully covered with new epithelium without any significant adverse reactions and PU dressings give much rapid and better healing than medical gauze. Histological examination revealed that PU dressings suppress the infiltration of inflammatory cells and accelerate fibroblast proliferation. It was also demonstrated that PULA-alt- PEG exhibits obvious better healing effect than PULA-ran-PEG does. This study has demonstrated that without further modification, plain alternating block polyurethane scaffold would help wound recovery efficiently. (C) 2016 Wiley Periodicals, Inc.